Real-time quantitative analysis of the influence of blue light on citrinin biosynthetic gene cluster expression in Monascus

被引:0
作者
Changlu Wang
Hua Yang
Mianhua Chen
Yurong Wang
Fengjuan Li
Cheng Luo
Shuyi Zhao
Dong He
机构
[1] Ministry of Education,Key Laboratory of Food Nutrition and Safety
[2] College of Food Engineering and Biotechnology,undefined
[3] Tianjin University of Science & Technology,undefined
来源
Biotechnology Letters | 2012年 / 34卷
关键词
Blue light; Citrinin; Monascus; RT-qPCR;
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摘要
When Monascus MX was grown under blue light instead of in the dark, citrinin production increased from 478 mg l−1 to 698 mg l−1. To explain this, the expression of the pksCT gene, which encodes citrinin polyketide synthase, and of 5 ORFs around it, were monitored. Blue light enhanced citrinin production by upregulating the expression of orf1, orf3, and orf4, indicating that pksCT was not the key gene responsible for the quantity of citrinin production in blue light.
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页码:1745 / 1748
页数:3
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