High-resolution melting analysis of ADAMTS18 methylation levels in gastric, colorectal and pancreatic cancers

被引：0

作者：

Zhi Li

Wei Zhang

Yong Shao

Chao Zhang

Qi Wu

Hong Yang

Xiangbin Wan

Jie Zhang

Ming Guan

Jun Wan

Bo Yu

机构：

[1] Basic Medical College of Zhengzhou University,Department of Histology and Embryology

[2] Henan Province Tumor Hospital,Department of General Surgery

[3] Ji-Nan University,HKUST

[4] Shenzhen-PKU-HKUST Medical Center,JNU Joint Lab

[5] Shenzhen Hospital Peking University,Biomedical Research Institute

[6] Huashan Hospital,Department of Dermatology

[7] Fudan University,Department of Laboratory Medicine, Central Laboratory

[8] The Hong Kong University of Science and Technology,Department of Biochemistry

来源：

Medical Oncology | 2010年 / 27卷

关键词：

ADAMTS18; Methylation; HRM; Gastric cancer; Colorectal cancer; Pancreatic cancer;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A disintegrin and metalloprotease with trombospondin motifs (ADAMTS) is a family of proteins characterized by the presence of a metalloproteinase domain linked to a variety of specialized ancillary domains. ADAMTS18 is a putative tumor suppressive gene related to nasopharyngeal carcinoma. We used high-resolution melting (HRM) analysis to detect the methylation levels of ADAMTS18 gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers, and equal number of adjacent normal tissues. The frequency of ADAMTS18 methylation in all three types of cancers was significantly higher than that in normal tissues. Expression levels of ADAMTS18 were inversely correlated with methylation levels. No significant association was found between ADAMTS18 methylation status and TNM staging in the cancers. In summary, epigenetic regulation of ADAMTS18 was associated with carcinogenesis. The application of HRM analysis is a fast and high-throughput way to investigate the epigenetic status of ADAMTS18.

引用

页码：998 / 1004

页数：6

共 64 条

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