Subcellular distribution of thallium: Morphological and quantitative study in rat myocardium

被引：0

作者：

Fukumoto M. ^{[1
,2
]}

Yoshida D. ^{[1
]}

Yoshida S. ^{[1
]}

机构：

[1] Department of Radiology, Kochi Medical School, Kohasu, Okoh, Nankoku

来源：

Annals of Nuclear Medicine | 1997年 / 11卷 / 4期

关键词：

Atomic absorption spectroscopy; Electron microscopy; Mitochondria; Myocardial cell; Thallium;

D O I：

10.1007/BF03165296

中图分类号：

学科分类号：

摘要：

The purpose of this study is to determine the subcellular distribution of thallium (SDTl) by electron microscopy and a newly designed fixation method that makes insoluble grains of Tl visible. Methods: To obtain the high dose necessary for electron microscopic visualization, we employed TlCl instead of 201TlCl. EM was performed in fixed rat myocardium resected at 20 min (early phase) and 3 hr (delay phase) after intravenous injection of TlCl. To fix Tl in the cell, we used orthovanadate in our fixative. Atomic absorption spectroscopy (AAS) of Tl and quantification of subcellular distribution of 201Tl (SD201Tl) were studied to prove the propriety of our fixation. Results: AAS detected Tl in the Tl-loaded specimen but not in the control, indicating that Tl was the origin of the grains observed in the former. In the early phase, numerous grains were observed in mitochondria, sarcoplasmic reticulum (SR), myofibrils, and nuclei, but no such grains were visible in controls. In the delay phase, grains were retained in mitochondria, SR and nuclei, but not in myofibrils. Electron microscopic SDTl (%) correlated with SD201Tl(%) calculated from isolated fractions. Conclusion: In both the early and delay phases, mitochondria are the major site of Tl and 201Tl uptake.

引用

页码：291 / 297

页数：6

共 29 条

[1] Mullins L.J., Moore R.D., The movement of thallium ions in muscle, J Gen Physiol, 43, pp. 759-773, (1960)
[2] Gehring P.J., Hammond P.B., The uptake of thallium by rabbit erythrocytes, J Pharmacol Exp Ther, 200, pp. 215-221, (1964)
[3] Britten J.S., Blank M., Thallium activation of the (Na+-K+) activated ATPase of the rabbit kidney, Biochim Biophys Acta, 159, pp. 160-166, (1968)
[4] Hasan M., Chandra S.V., Bajpai V.K., Ali S.F., Electron microscopic effects of thallium poisoning on the rat hypothalamus and hypocampus: Biochemica-changes in the cerebrum, Brain Res Bull, 2, pp. 255-261, (1977)
[5] Hasan M., Electron microscopic study of the effects of thallium poisoning on the rat cerebellum, Forensic Sci Int, 11, pp. 139-146, (1978)
[6] Woods J.S., Fowler B.A., Alteration of hepatocellular structure and function by thallium chloride: Ultrastructural, morphometric and biochemical studies, Toxicol Appl Pharmacol, 83, pp. 218-229, (1986)
[7] Fox J., Conductance and selectivity properties of a substate of the rabbit sarcoplasmic reticulum channel, Biophys J, 47, pp. 573-576, (1985)
[8] Fox J., Thallous ion permeation through the cation selective channel of the sarcoplasmic reticulum: Anomalous mole fraction dependence, Biochim Biophys Acta, 736, pp. 241-245, (1983)
[9] Fox J., Experimental and theoretical studies on Tl+ interactions with the cation selective channel of the sarcoplasmic reticulum, J Membr Biol, 84, pp. 9-23, (1985)
[10] Brismar T., Lefvert A., Jondal M., A method for analysis of cellular K-transport mechanisms through thallium (201Tl) uptake in human lymphocytes, Acta Physiol Scand, 150, pp. 299-303, (1994)

← 1 2 3 →