Isolation and characterization of novel organic solvent-tolerant bacteria

被引:0
作者
Zileena Zahir
Kimberley D. Seed
Jonathan J. Dennis
机构
[1] University of Alberta,Department of Biological Sciences
来源
Extremophiles | 2006年 / 10卷
关键词
Solvent tolerance; Organic solvents; Bacterial cell envelope; Enrichment;
D O I
暂无
中图分类号
学科分类号
摘要
Some organic solvents are extremely toxic to living organisms by virtue of their ability to partition into and disrupt the normal functioning of biological membranes. In recent years, several bacteria have been discovered that are more tolerant to these toxic solvents than most microorganisms. Using enrichment procedures, we have isolated new organic solvent-tolerant bacteria from both hyrdocarbon-contaminated and pristine soil samples. These organisms were characterized by several different experimental procedures including description of their cellular physiology, 16S rDNA homology, organic solvent tolerance range, and survival after solvent exposure. The results indicate that gram-positive bacteria can be isolated from the environment that are as tolerant to toxic organic solvents, if not more so, than the most organic solvent-tolerant gram-negative bacteria.
引用
收藏
页码:129 / 138
页数:9
相关论文
共 112 条
[11]  
Lal B(1998)A J Bacteriol 180 6769-6772
[12]  
Cruden DL(1998) thrives in high concentration of toluene J Bacteriol 180 3692-3696
[13]  
Wolfram JH(1994)Bacteria tolerant to organic solvents Trends Microbiol 2 489-493
[14]  
Rogers RD(2002)Identification and molecular characterization of an efflux pump involved in J Biosci Bioeng 94 45-51
[15]  
Gibson DT(1993) S12 solvent tolerance J Ferment Bioeng 76 397-399
[16]  
Christensen LP(1992)Active efflux of organic solvents by Biosci Biotechnol Biochem 56 1872-1873
[17]  
Lam J(1994) S12 is induced by solvents Appl Environ Microbiol 60 3884-3886
[18]  
Davidson PM(1996)Isolation and characterization of toluene-sensitive mutants from the toluene-resistant bacterium J Gen Appl Microbiol 42 349-354
[19]  
Barnden AL(1990) GM73 Enzyme Microb Technol 12 281-291
[20]  
de Bont JAM(1997)Efflux pumps and drug resistance in gram-negative bacteria Microbiology 143 2975-2981