Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis

被引：0
作者：
Min Zhang
Cong Zhao
LianXiang Du
FuPing Lu
Chen Gao
机构：
[1] Shenyang Agricultural University,College of Engineering
[2] Tianjin University of Science and Technology,College of Biotechnology
[3] Nankai University,College of Life Science
来源：
Science in China Series C: Life Sciences | 2008年 / 51卷
关键词：
thermophilic neutral protease; expression; purification; characterization;
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摘要：
The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg−1. As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65°C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 °C.
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页码：52 / 59
页数：7
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