Discovery of tear biomarkers in children with chronic non-infectious anterior uveitis: a pilot study

被引：36

作者：

Angeles-Han S.T. ^{[1
,2
]}

Yeh S. ^{[3
]}

Patel P. ^{[3
]}

Duong D. ^{[4
]}

Jenkins K. ^{[5
]}

Rouster-Stevens K.A. ^{[5
,6
]}

Altaye M. ^{[2
,8
]}

Fall N. ^{[1
,2
]}

Thornton S. ^{[1
,2
]}

Prahalad S. ^{[5
,6
,7
]}

Holland G.N. ^{[9
]}

机构：

[1] Division of Rheumatology, Cincinnati Children’s Hospital Medical Center, 3333 Burnet Avenue, MLC 4010, Cincinnati, 45229, OH

[2] Department of Pediatrics, University of Cincinnati, Cincinnati, OH

[3] Department of Ophthalmology, Emory Eye Center, Emory University School of Medicine, 1365 Clifton Rd B, Atlanta, 30322, GA

[4] Emory Integrated Proteomics Core, Emory University, 1510 Clifton Rd, Atlanta, 30322, GA

[5] Childrens Healthcare of Atlanta, Emory Children’s Center, 2015 Uppergate Drive, Atlanta, 30322, GA

[6] Department of Pediatrics, Emory University School of Medicine, Atlanta, GA

[7] Department of Human Genetics, Emory University School of Medicine, Atlanta, GA

[8] Division of Biostatistics and Epidemiology, Cincinnati Children’s Hospital Medical Center, University of Cincinnati, 3333 Burnet Avenue, MLC 5041, Cincinnati, 45229, OH

[9] UCLA Stein Eye Institute and David Geffen School of Medicine at University of California, 100 Stein Plaza, Los Angeles, 90095-7000, CA

来源：

Journal of Ophthalmic Inflammation and Infection | / 8卷 / 1期

关键词：

Biomarkers; Juvenile idiopathic arthritis; Uveitis;

D O I：

10.1186/s12348-018-0156-5

中图分类号：

学科分类号：

摘要：

Background: Biomarkers in easily obtained specimens that accurately predict uveitis in children with juvenile idiopathic arthritis (JIA) are needed. Aqueous humor has been studied for biomarkers, but is not routinely available. We evaluated tears from children with chronic anterior uveitis (CAU) for biomarkers reported in aqueous humor. In this pilot study, we used Schirmer strips to collect tears from seven children (nine eyes); three children had JIA- associated uveitis (JIA-U) and four had idiopathic disease (I-CAU). Liquid chromatography-tandem mass spectrometry was used to identify and quantify tear proteins. The Mann-Whitney U test identified differential tear protein expression between children with JIA-U and those with I-CAU. Results: S100A9, LAP3, TTR, MIF, sCD14, S100A8, and SAA1 were detected in tears of all children; the same cytokines have been reported in aqueous humor of children with JIA-U. Tears from children with JIA-U had higher expression of proteins associated with inflammatory arthritis (SEMA3G, TIMP1, HEXB, ERN1, and SAA1) than tears from those with I-CAU. In addition, we found higher expression of sCD14, S100A8, and SAA1, but lower expression of S100A9, LAP3, TTR, and MIF, in tears from children with JIA-U compared to tears from those with I-CAU. Conclusions: Tears contain similar cytokine profiles to aqueous humor in children with CAU and may be a clinically useful source of disease biomarkers. Tears from children with JIA-U also contain cytokines associated with inflammatory arthritis; furthermore, differential expression of other tear proteins as well may provide clues to intrinsic differences between JIA-U and I-CAU, despite their similar clinical phenotypes. © 2018, The Author(s).

引用

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