In vitro propagation of spine gourd (Momordica dioica Roxb.) and assessment of genetic fidelity of micropropagated plants using RAPD analysis

被引：15
作者：
Govind Kumar Rai
Major Singh
Neha Prakash Rai
D. R. Bhardwaj
Sanjeev Kumar
机构：
[1] Indian Institute of Vegetable Research, Varanasi 221305, P. B. No. 01, P.O. Jakhini (Shahanshahpur)
来源：
Physiology and Molecular Biology of Plants | 2012年 / 18卷 / 3期
关键词：
Axillary shoot proliferation; Dioecious; Genetic fidelity; Micropropagation; Momordica dioica; Nodal explants;
D O I：
10.1007/s12298-012-0109-7
中图分类号：
学科分类号：
摘要：
An efficient protocol for rapid in vitro clonal propagation of spine gourd (Momordica dioica Roxb.) genotype RSR/DR15 (female) and DR/NKB-28 (male) was developed through enhanced axillary shoot proliferation from nodal segments. Maximum shoot proliferation of 6. 2 shoots per explant with 100 % shoot regeneration frequency was obtained from the female genotype on Murashige and Skoog's (1962) medium supplemented with 0. 9 μM N6-benzyladenine (BA) and 200 mg l -1 casein hydrolysate (CH). While from the male genotype the optimum shoot regeneration frequency (86. 6 %) and 6. 4 shoots per explant was obtained on MS medium supplemented with 2. 2 μM BA. CH induced vigorous shoots, promoted callus formation, and proved inhibitory for shoot differentiation and shoot length, especially in explants from male genotype. Rooting was optimum on half-strength MS medium (male 92. 8 %, female 74. 6 %) containing 4. 9 μM indole-3-butyric acid (IBA). Plantlets were transferred to plastic cups containing a mixture of cocopit and perlite (1:1 ratio) and then to soil after 2-3 weeks. 84 % female and 81 % male regenerated plantlets survived and grew vigorously in the field. Genetic stability of the regenerated plants was assessed using random amplified polymorphic DNA (RAPD). The amplification products were monomorphic in the in vitro propagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic integrity of in vitro propagated plants. This micropropagation procedure could be useful for raising genetically uniform planting material of known sex for commercial cultivation or build-up of plant material of a specific sex-type. © 2012 Prof. H.S. Srivastava Foundation for Science and Society.
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页码：273 / 280
页数：7
相关论文
共 29 条
  • [1] Agrawal V., Prakash S., Gupta S.C., Altman A., Ziv M., Izhar S., Differential hormonal requirements for clonal propagation of male and female jojoba plants, Current Science and Biotechnology in Agriculture: Plant Biotechnology and in Vitro Biology in the 21st Century, pp. 23-26, (1999)
  • [2] Ali M., Okubo H., Fujii T., Fujieda K., Techniques for propagation and breeding of kakrol (Momordica dioica Roxb.), Sci Hortic, 47, 3-4, pp. 335-343, (1991)
  • [3] Ali M., Shrivastava V., Characterization of phytoconstitutents of the fruits of Momordica dioica, J Pharamaceutical Sci, 60, pp. 287-289, (1998)
  • [4] Ahmad N., Anis M., In vitro mass propagation of Cucumis sativus L. from nodal segments, Turk J Bot, 29, pp. 237-240, (2005)
  • [5] Andrade L.B., Echeverrigaray S., Fracaro F., Pauletti G.F., Rota L., The effect of growth regulators on shoot propagation and rooting of common lavender (Lavandula vera DC), Plant Cell Tiss Org Cult, 56, 2, pp. 79-83, (1999)
  • [6] Banerjee S., Zehra M., Kumar S., In vitro multiplication of Centella asiatica, a medicinal herb from leaf explants, Curr Sci, 76, pp. 147-148, (1999)
  • [7] Bharathi L.K., Naik G., Singh H.S., Dora D.K., Peter K.V., Spine gourd, Underutilized and Underexploited Horticultural Crops, pp. 289-295, (2007)
  • [8] Bopana N., Saxena S., In vitro propagation of a high value medicinal plant: Asparagus racemosus Willd, In Vitro Cell Dev Biol-Plant, 44, pp. 525-532, (2008)
  • [9] Carvalho L.C., Goulao L., Oliveira C., Goncalves J.C., Amancio S., RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids, Plant Cell Tiss and Org Cult, 77, pp. 23-27, (2004)
  • [10] Ceasar S.A., Maxwell S.L., Prasad K.B., Karthigan M., Ignacimuthu S., Highly efficient shoot regeneration of Bacopa monnieri L. using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD, Acta Physiol Plant, 32, pp. 443-452, (2010)
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