In-depth characterization of a novel live-attenuated Mayaro virus vaccine candidate using an immunocompetent mouse model of Mayaro disease

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作者
Mânlio Tasso de Oliveira Mota
Vivian Vasconcelos Costa
Michelle Amantéa Sugimoto
Georgia de Freitas Guimarães
Celso Martins Queiroz-Junior
Thaiane Pinto Moreira
Carla Daiane de Sousa
Franciele Martins Santos
Victoria Fulgêncio Queiroz
Ingredy Passos
Josy Hubner
Danielle Gloria Souza
Scott C. Weaver
Mauro Martins Teixeira
Maurício Lacerda Nogueira
机构
[1] Faculdade de Medicina de São José do Rio Preto,
[2] São José do Rio Preto,undefined
[3] Institute of Biological Sciences,undefined
[4] Universidade Federal de Minas Gerais,undefined
[5] World Reference Center for Emerging Viruses and Arboviruses,undefined
[6] Institute for Human Infections and Immunity,undefined
[7] and Department of Microbiology and Immunology,undefined
[8] University of Texas Medical Branch,undefined
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Scientific Reports | / 10卷
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摘要
Mayaro virus (MAYV) is endemic in South American countries where it is responsible for sporadic outbreaks of acute febrile illness. The hallmark of MAYV infection is a highly debilitating and chronic arthralgia. Although MAYV emergence is a potential threat, there are no specific therapies or licensed vaccine. In this study, we developed a murine model of MAYV infection that emulates many of the most relevant clinical features of the infection in humans and tested a live-attenuated MAYV vaccine candidate (MAYV/IRES). Intraplantar inoculation of a WT strain of MAYV into immunocompetent mice induced persistent hypernociception, transient viral replication in target organs, systemic production of inflammatory cytokines, chemokines and specific humoral IgM and IgG responses. Inoculation of MAYV/IRES in BALB/c mice induced strong specific cellular and humoral responses. Moreover, MAYV/IRES vaccination of immunocompetent and interferon receptor-defective mice resulted in protection from disease induced by the virulent wt MAYV strain. Thus, this study describes a novel model of MAYV infection in immunocompetent mice and highlights the potential role of a live-attenuated MAYV vaccine candidate in host’s protection from disease induced by a virulent MAYV strain.
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[1]  
Coimbra TL(2007)Mayaro virus: imported cases of human infection in Sao Paulo State, Brazil Rev. do Inst. de. Medicina Tropical de Sao Paulo 49 221-224
[2]  
Anderson CR(1957)Mayaro virus: a new human disease agent. II. Isolation from blood of patients in Trinidad, B.W.I Am. J. tropical Med. Hyg. 6 1012-1016
[3]  
Downs WG(2012)Mayaro virus infection in traveler returning from Amazon Basin, northern Peru Emerg. Infect. Dis. 18 695-696
[4]  
Wattley GH(2004)Family cluster of Mayaro fever, Venezuela Emerg. Infect. Dis. 10 1304-1306
[5]  
Ahin NW(1957)Mayaro virus: a new human disease agent. III. Investigation of an epidemic of acute febrile illness on the river Guama in Para, Brazil, and isolation of Mayaro virus as causative agent Am. J. tropical Med. Hyg. 6 1017-1023
[6]  
Reese AA(1981)An outbreak of Mayaro virus disease in Belterra, Brazil. I. Clinical and virological findings Am. J. tropical Med. Hyg. 30 674-681
[7]  
Neumayr A(1981)An outbreak of Mayaro virus disease in Belterra, Brazil. II. Epidemiology Am. J. tropical Med. Hyg. 30 682-688
[8]  
Torres JR(2016)Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiabá, state of Mato Grosso, Brazil Mem. Inst. Oswaldo Cruz 111 20-29
[9]  
Causey OR(2015)Neutralising antibodies for Mayaro virus in Pantanal, Brazil Mem. Inst. Oswaldo Cruz 110 125-133
[10]  
Maroja OM(2014)Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil Mem. Inst. Oswaldo Cruz 109 820-823