Ion mobility–mass spectrometry as a tool to investigate protein–ligand interactions

被引：0
作者：
Melanie Göth
Kevin Pagel
机构：
[1] Freie Universität Berlin,Institute of Chemistry and Biochemistry
[2] Fritz-Haber-Institut der Max-Planck-Gesellschaft,Department of Molecular Physics
来源：
Analytical and Bioanalytical Chemistry | 2017年 / 409卷
关键词：
Ion mobility–mass spectrometry; Protein–ligand complexes; Native mass spectrometry; Noncovalent complexes; Catch and release; Collision-induced unfolding;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Ion mobility–mass spectrometry (IM-MS) is a powerful tool for the simultaneous analysis of mass, charge, size, and shape of ionic species. It allows the characterization of even low-abundant species in complex samples and is therefore particularly suitable for the analysis of proteins and their assemblies. In the last few years even complex and intractable species have been investigated successfully with IM-MS and the number of publications in this field is steadily growing. This trend article highlights recent advances in which IM-MS was used to study protein–ligand complexes and in particular focuses on the catch and release (CaR) strategy and collision-induced unfolding (CIU).
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页码：4305 / 4310
页数：5
相关论文
共 203 条
  • [1] Marcoux J(2013)Twenty years of gas phase structural biology Structure 21 1541-1550
  • [2] Robinson CV(2016)von Helden G. Retention of native protein structures in the absence of solvent: a coupled ion mobility and spectroscopic study Angew Chem Int Ed 55 14173-14176
  • [3] Seo J(1996)Probing the nature of noncovalent interactions by mass spectrometry. A study of protein−CoA ligand binding and assembly J Am Chem Soc 118 8646-8653
  • [4] Hoffmann W(2009)Hydrophobic protein−ligand interactions preserved in the gas phase J Am Chem Soc 131 15980-15981
  • [5] Warnke S(2012)Do charge state signatures guarantee protein conformations? J Am Soc Mass Spectrom 23 1161-1168
  • [6] Bowers MT(2002)Quantitative determination of noncovalent binding interactions using soft ionization mass spectrometry Int J Mass Spectrom 216 1-27
  • [7] Pagel K(2012)Reliable determinations of protein–ligand interactions by direct ESI-MS measurements. Are we there yet? J Am Soc Mass Spectrom 23 431-441
  • [8] Robinson CV(2010)Ion mobility mass spectrometry of proteins and protein assemblies Chem Soc Rev 39 1633-1655
  • [9] Chung EW(2013)Unraveling the mechanism of electrospray ionization Anal Chem 85 2-9
  • [10] Kragelund BB(1958)Mobility of gaseous lons in weak electric fields Ann Phys 4 233-270
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