Identification of Pseudomonas syringae pv. phaseolicola as the causal agent of halo blight in yellow beans in northern Sinaloa, Mexico

被引:0
作者
R. Félix-Gastélum
I. E. Maldonado-Mendoza
R. Navarrete-Maya
N. G. Olivas-Peraza
H. Brito-Vega
J. A. Acosta-Gallegos
机构
[1] Universidad de Occidente,Departamento de Ciencias Biológicas
[2] Unidad Los Mochis,Departamento de Biotecnología Agrícola, Instituto Politécnico Nacional
[3] CIIDIR-Unidad Sinaloa,undefined
[4] Universidad Nacional Autónoma de México,undefined
[5] Facultad de Estudios Superiores Cuautitlán,undefined
[6] UNIGRAS,undefined
[7] Campo Experimental Bajío,undefined
[8] INIFAP,undefined
[9] SAGARPA,undefined
来源
Phytoparasitica | 2016年 / 44卷
关键词
Bacterial disease; Physiological races; and ; genes;
D O I
暂无
中图分类号
学科分类号
摘要
Yellow beans are produced under irrigation during the fall-winter growing season in the coastal lowlands of Sinaloa, Mexico. Halo blight, is the most important disease during the winter time. The objectives of this study were to: a) identify the causal agent of the disease and b) determine the physiological races of the bacterium. Twelve isolates of the bacterium associated with infected leaves and pods from the Azufrado Higuera cultivar were obtained from December 2013 through January 2014. Biochemical, physiological and molecular characteristics of the bacterial isolates coincide with those of Pseudomonas syringae pv. phaseolicola (Psph) reported in the highlands of Mexico. All twelve isolates of the bacterium from bean were inoculated under greenhouse conditions and caused similar symptoms as those observed in the field. The genes rpoB and rpoD were sequenced for phylogenetic analysis and compared to databases to confirm their identities. These markers were thus used to determine that the 12 analyzed isolates are identical, and are similar to the Psph sequences currently available in databases. Compatibility patterns between bean cultivars from the differential set revealed that six out of the twelve isolates infected all differential plants and matched the pattern of race six. In contrast, the remaining isolates displayed inconclusive compatibility patterns, indicating that the differential set does not provide conclusive information for pathotype identification. This suggests that the current differential set of genotypes requires the inclusion of novel bean genotypes, in order to explore the existence of potential new races of this pathogen.
引用
收藏
页码:369 / 378
页数:9
相关论文
共 118 条
[1]  
Akhavan A(2013)Bean common bacterial blight: pathogen epiphytic life and effect of irrigation practices Springer Plus 2 41-627
[2]  
Bahar M(2011) pv. Molecular Plant Pathology 12 617-54
[3]  
Askarian H(2016): from ‘has bean’ to supermodel Canadian Journal of Plant Pathology 38 41-182
[4]  
Reza Lak M(2011)Identification of bacterial pathogens and races of Sydowia 63 169-166
[5]  
Nazemi A(1989) pv. Cladistics 5 164-854
[6]  
Zamani Z(2015) from dry bean fields in Western Canada Frontiers in Microbiology 6 214-242
[7]  
Arnold DL(2000)Powdery mildew on common bean ( Applied and Environmental Microbiology 66 850-20
[8]  
Lovell HC(2014) L.) in Northern Sinaloa, Mexico International Journal of Virology 10 235-307
[9]  
Jackson RW(1980)Phylip – phylogeny inference package (version 3.2) Journal of Molecular Evolution 16 111-2108
[10]  
Mansfield JW(1954)Phylogenomics and systematics in Journal of Laboratory and Clinical Medicine 44 301-208