Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L.

被引：0

作者：

A. Ohara

Y. Akasaka

H. Daimon

M. Mii

机构：

[1] Hokkaido National Agricultural Experiment Station,

[2] Shinsei,undefined

[3] Memuro,undefined

[4] Kasai,undefined

[5] Hokkaido 0820071,undefined

[6] Japan,undefined

[7] Faculty of Horticulture,undefined

[8] Chiba University,undefined

[9] Matsudo,undefined

[10] Chiba 2718510,undefined

[11] Japan e-mail: mii@midori.h.chiba-u.ac.jp Fax: +81-47-3088852,undefined

[12] College of Agriculture,undefined

[13] Osaka Prefecture University,undefined

[14] Sakai,undefined

[15] Osaka 5998531,undefined

[16] Japan,undefined

来源：

Plant Cell Reports | 2000年 / 19卷

关键词：

Key words Agrobacterium rhizogenes; Crotalaria juncea; Plant regeneration; Transformation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Hairy roots were induced from leaf segments of Crotalaria juncea, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.

引用

页码：563 / 568

页数：5