Development of 81 new polymorphic EST-derived microsatellite markers for the olive flounder, Paralichthys olivaceus

被引:0
作者
Woo-Jin Kim
Young-Ok Kim
Bo-Hye Nam
Hee Jeong Kong
Eun-Mi Park
Jung-Ha Kang
Jeong-Ho Lee
Kyung-Kil Kim
机构
[1] National Fisheries Research and Development Institute,Biotechnology Research Institute
[2] National Fisheries Research and Development Institute,Genetics & Breeding Research Center
来源
Conservation Genetics | 2009年 / 10卷
关键词
Olive flounder; EST; Microsatellites; Segregation analysis; Pedigree tracing;
D O I
暂无
中图分类号
学科分类号
摘要
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 81 polymorphic microsatellite markers from 4,940 ESTs of the olive flounder, Paralichthys olivaceus. Out of 100 EST-derived microsatellites for which PCR primers were designed, 81 loci were polymorphic in 30 individuals from a single natural population with 2–28 (mean 10.6) alleles per locus. The observed and expected heterozygosities of these loci were 0.033–1.000 and 0.033–0.965, respectively. Segregation analysis within a mapping family revealed non-amplifying null alleles at five loci. These new EST-derived microsatellite markers should be useful for population genetic analyses, pedigree tracing and constructing a linkage map for olive flounder.
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页码:1105 / 1111
页数:6
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