Isolation and characterization of microsatellite loci in Rhinogobio ventralis

被引：0
作者：
Nian Xu
Fang Shi
Meihua Xiong
Yanfu Que
Zhong Yang
Bin Zhu
Jianbo Chang
机构：
[1] Chinese Academy of Sciences,Institute of Hydrobiology
[2] Ministry of Water Resources and Chinese Academy of Sciences,Institute of Hydroecology
[3] The Graduate School of the Chinese Academy of Sciences,undefined
来源：
Conservation Genetics Resources | 2010年 / 2卷
关键词：
Microsatellites; Polymorphic loci; Population genetics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
From (AAAG)n enriched genomic library, we developed 25 microsatellite loci in Rhinogobio ventralis, an endemic fish in the upper reaches of the Yangtze River in China, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Twelve primer pairs exhibited polymorphic with two to 11 alleles per locus, while the rest 13 displayed monomorphic in a test on a population of 40 individuals. Observed heterozygosities ranged from 0.15 to 0.88. Nine loci are highly informative (PIC > 0.5). These loci should be useful in further genetic analyses in different populations of Rhinogobio ventralis and its congeneric species.
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页码：1 / 3
页数：2
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