Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis

被引：0
|
作者：
V. G. Sunnichan
K. R. Shivanna
H. Y. Mohan Ram
机构：
[1] Department of Botany,
[2] University of Delhi,undefined
[3] Delhi 110 007,undefined
[4] India,undefined
[5] Department of Environmental Biology,undefined
[6] University of Delhi South Campus,undefined
[7] Benito Juarez Road,undefined
[8] New Delhi 110 021,undefined
[9] India,undefined
来源：
Plant Cell Reports | 1998年 / 17卷
关键词：
Key words Gum karaya; Somatic embryogenesis; Sterculia urens; Thidiazuron; Tree tissue culture;
D O I：
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学科分类号：
摘要：
Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μm N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μm indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μm 2,4-dichlorophenoxyacetic acid and 8.90 μm BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μm thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to 1/4-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field.
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页码：951 / 956
页数：5
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