Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis

被引：0
|
作者：
V. G. Sunnichan
K. R. Shivanna
H. Y. Mohan Ram
机构：
[1] Department of Botany,
[2] University of Delhi,undefined
[3] Delhi 110 007,undefined
[4] India,undefined
[5] Department of Environmental Biology,undefined
[6] University of Delhi South Campus,undefined
[7] Benito Juarez Road,undefined
[8] New Delhi 110 021,undefined
[9] India,undefined
来源：
Plant Cell Reports | 1998年 / 17卷
关键词：
Key words Gum karaya; Somatic embryogenesis; Sterculia urens; Thidiazuron; Tree tissue culture;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μm N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μm indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μm 2,4-dichlorophenoxyacetic acid and 8.90 μm BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μm thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to 1/4-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field.
引用
收藏
页码：951 / 956
页数：5
相关论文
共 47 条
  • [31] Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida L 'Moneyway'
    vanderSalm, TPM
    vanderToorn, CJG
    tenCate, CHH
    Dons, HJM
    PLANT CELL REPORTS, 1996, 15 (07) : 522 - 526
  • [32] Micropropagation of Selected Trees of Arbutus unedo L. through Axillary Shoot Proliferation and Somatic Embryogenesis
    Gomes, F.
    Lopes, M. L.
    Santos, T.
    Canhoto, J. M.
    I INTERNATIONAL SYMPOSIUM ON BIOTECHNOLOGY OF FRUIT SPECIES: BIOTECHFRUIT2008, 2009, 839 : 111 - 116
  • [33] Improving the Efficiency of Adventitious Shoot Induction and Somatic Embryogenesis via Modification of WUSCHEL and LEAFY COTYLEDON 1
    Ikeda, Miho
    Takahashi, Mikiya
    Fujiwara, Sumire
    Mitsuda, Nobutaka
    Ohme-Takagi, Masaru
    PLANTS-BASEL, 2020, 9 (11): : 1 - 11
  • [34] COTYLEDONARY NODE CULTURE AND MULTIPLE SHOOT FORMATION IN PEANUT - EVIDENCES FOR SOMATIC EMBRYOGENESIS
    BANERJEE, S
    BANDYOPADHYAY, S
    GHOSH, PD
    CURRENT SCIENCE, 1988, 57 (05): : 252 - 255
  • [35] MICROPROPAGATION OF FASTIGIATE BIRD CHERRY (PRUNUS-PADUS L) AND ADVENTITIOUS SHOOT FORMATION FROM LEAVES
    HAMMATT, N
    JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 1993, 68 (06): : 975 - 981
  • [36] Direct primary somatic embryogenesis and shoot formation from immature leaves of Manihot esculenta
    Ma, GH
    Xu, QS
    Xian, YL
    ACTA BOTANICA SINICA, 1998, 40 (06): : 503 - +
  • [37] ADVENTITIOUS SHOOT INDUCTION AND SOMATIC EMBRYOGENESIS WITH INTACT SEEDLINGS OF SEVERAL HYBRID SEED GERANIUM (PELARGONIUM X HORTORUM BAILEY) VARIETIES
    QURESHI, JA
    SAXENA, PK
    PLANT CELL REPORTS, 1992, 11 (09) : 443 - 448
  • [38] IN VITRO REGENERATION OF SOPHORA JAPONICA L. 'PENDULA' FROM COTYLEDON EXPLANTS VIA SOMATIC EMBRYOGENESIS AND ADVENTITIOUS SHOOT INDUCTION
    Cao, Xiao-yan
    Wang, Zhe-zhi
    Dong, Niu
    PROPAGATION OF ORNAMENTAL PLANTS, 2009, 9 (02): : 71 - 77
  • [39] Rapid micropropagation through direct somatic embryogenesis and bulbil formation from leaf explants in Curculigo orchioides
    Suri, Sarahjeer S.
    Arora, Dilip K.
    Sharma, Rachana
    Ramawat, K.G.
    Indian Journal of Experimental Biology, 1998, 36 (11): : 1130 - 1135
  • [40] STUDIES ON REGENERATION FROM SOMATIC TISSUE INVITRO .1. ADVENTITIOUS SHOOT FORMATION FROM APPLE LEAF EXPLANTS
    HANKE, V
    ROHDE, A
    GRAFE, C
    GARTENBAUWISSENSCHAFT, 1991, 56 (05): : 214 - 220
12345