Assignment of 280 swine genomic inserts including 31 microsatellites from BAC clones to the swine RH map (IMpRH map)

被引：8

作者：

Kiuchi S. ^{[1
]}

Inage Y. ^{[2
]}

Hiraiwa H. ^{[1
]}

Uenishi H. ^{[1
]}

Yasue H. ^{[1
]}

机构：

[1] Department of Genome Research, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Kukisaki-machi

[2] Animal Genome Program Team, STAFF Institute, Tsukuba, Ibaraki 305-0854, 446-1, Ippaizuka

来源：

Mammalian Genome | 2002年 / 13卷 / 2期

关键词：

D O I：

10.1007/s0033501-2099-6

中图分类号：

学科分类号：

摘要：

A precise genetic map containing anonymous markers and genes is indispensable for the efficient selection of candidate gene(s) responsible for quantitative trait loci (QTL) traits. For this purpose, a first version of a radiation hybrid cell (RH) map has been constructed by using the INRA-University of Minnesota RH panel for 757 markers (IMpRH) (Hawken et al. 1999, Mamm. Genome 10: 824-830). In this study, 280 swine genomic fragments in BAC clones were assigned to the IMpRH map; 255 BAC clones were successfully linked to first-generation linkage groups (LOD > 4.8). The remaining 25 clones could not be mapped, because their lod-scores to the closest markers in the first generation map were less than 4.8. In addition, 16 BAC clones, mapped to swine Chromosome (Chr) 1 by IMpRH mapping, were subjected to isolation of microsatellites (MSs). Thirty-one MSs were isolated from 15 BAC clones, and 24 of 31 (77%) MSs derived from 14 clones were found to be polymorphic. We also mapped both termini of 12 BAC clones to the IMpRH map, in order to measure resolution of the IMpRH map; the resolution was found to range from 8 kb/centiRay to more than 126 kb/centiRay depending on the region.

引用

页码：80 / 88

页数：8

共 30 条

[1]

Andersson L., Haley C.S., Ellegren H., Knott S.A., Johansson M., Et al., Genetic mapping of quantitative trait loci for growth and fatness in pigs, Science, 263, pp. 1771-1774, (1994)

[2]

Archibald A.L., Haley C.S., Brown J.F., Couperwhite S., McQueen H.A., Et al., The PiGMaP consortium linkage map of the pig (Sus scrofa), Mamm Genome, 6, pp. 157-175, (1995)

[3]

Boehnke M., Lunetta K., Hauser E., Lange K., Uro J., Et al., RHMAP 3.0, (1996)

[4]

Buitkamp J., Kollers S., Durstewitz G., Fries R., Welzel K., Et al., Construction and characterization of a gridded cattle BAC library, Anim Genet, 31, pp. 347-351, (2000)

[5]

De Koning D.J., Janss L.L., Rattink A.P., Van Oers P.A., De Vries B.J., Et al., Detection of quantitative trait loci for backfat thickness and intramuscular fat content in pigs (Sus scrofa), Genetics, 152, pp. 1679-1690, (1999)

[6]

De Koning D.J., Rattink A.P., Harlizius B., Van Arendonk J.A., Brascamp E.W., Et al., Genome-wide scan for body composition in pigs reveals important role of imprinting, Proc Natl Acad Sci USA, 97, pp. 7947-7950, (2000)

[7]

Edfors-Lilja I., Wattrang E., Marklund L., Moller M., Andersson-Eklund L., Et al., Mapping quantitative trait loci for immune capacity in the pig, J Immunol, 161, pp. 829-835, (1998)

[8]

Foster J.W., Schafer A.J., Critcher R., Spillett D.J., Feakes R.W., Et al., A high-resolution whole genome radiation hybrid map of human chromosome 17q22-q25.3 across the genes for GH and TK, Genomics, 33, pp. 185-192, (1996)

[9]

Genet C., Renard C., Cabau C., Rogel-Gaillard C., Gellin J., Et al., In the QTL region surrounding porcine MHC, gene order is conserved with human genome, Mamm Genome, 12, pp. 246-249, (2001)

[10]

Hawken R.J., Murtaugh J., Flickinger G.H., Yerle M., Robic A., Et al., A first-generation porcine whole-genome radiation hybrid map, Mamm Genome, 10, pp. 824-830, (1999)

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