Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. under salt stress

被引:1
|
作者
E. N. Baranova
E. K. Nodel’man
L. V. Kurenina
A. A. Gulevich
G. B. Baranova
L. R. Bogoutdinova
E. N. Akanov
M. R. Khaliluev
机构
[1] All-Russia Research Institute of Agricultural Biotechnology,
[2] Williams All-Russia Fodder Research Institute,undefined
[3] Pryanishnikov All-Russia Research Institute of Agricultural Chemistry,undefined
[4] Russian State Agrarian University—Moscow Timiryazev Agricultural Academy,undefined
关键词
Fe-dependent superoxide dismutase; salt stress; ultrastructural organization of chloroplasts and mitochondria;
D O I
10.3103/S1068367414060056
中图分类号
学科分类号
摘要
Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. with the pea rbcS gene leader sequence under chloride and sulfate salinity has been studied. Dark respiration of transgenic tomato regenerants under stress conditions has been estimated. It has been established that expression of the FeSOD1 gene in transgenic tomato plants under salinity leads to a decrease in damage of ultrastructure organization of plastids and mitochondria and affects the size and shape of their inclusions, suggesting a protective effect of this gene under salinity. The obtained experimental data show the effectiveness of using a genetic engineering strategy of redirection and subsequent accumulation of cytoplasmic Fe-dependent SOD from Arabidopsis thaliana (L.) Heynh. in chloroplasts for increasing the tolerance of crops to salt stress.
引用
收藏
页码:426 / 431
页数:5
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