Cloning and DNA-binding properties of ethylene response factor, LeERF1 and LeERF2, in tomato

被引：0
|
作者：
Zhang Hongxing
Zhu Benzhong
Yu Bianyun
Hao Yanling
Fu Daqi
Xu Wentao
Luo Yunbo
机构：
[1] China Agricultural University,Department of Biotechnology, the College of Food Science and Nutritional Engineering
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
ethylene response factor; GCC box; tomato;
D O I：
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中图分类号：
学科分类号：
摘要：
Two new genes, LeERF1 andLeERF2, were isolated from a tomato (Lycopersicon esculentum cv. Lichun) cDNA library. Phylogenetic analysis indicated that they encoded Ethylene Responsive Element Binding Proteins (EREBPs), characterized by a conserved ERF (ethylene response factor) domain of specific binding plant cis-acting elements GCC box. Both LeERF1 and LeERF2 proteins were obtained via prokaryotic expression and purification. Electrophoretic mobility shift assay showed that LeERF1 and LeERF2 protein could bind to the promoter of the NP24 gene coding for pathogenesis-related protein osmotin precursor but not the mutant promoter where its GCC box was deleted. Polyclonal antibodies of LeERF1 and LeERF2 blocked their binding in vitro.
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页码：423 / 428
页数：5
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