Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honey

被引：0

作者：

Eremin, S. A. ^{[1
,2
]}

Mukhametova, L. I. ^{[1
]}

Arutyunyan, D. A. ^{[1
]}

Tereshchenkov, A. G. ^{[3
]}

Sumbatyan, N. V. ^{[1
]}

Priima, A. D. ^{[4
]}

Nesterenko, I. S. ^{[4
]}

Berlina, A. N. ^{[2
]}

Sotnikov, D. V. ^{[2
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia

[2] Russian Acad Sci, Bach Inst Biochem, Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia

[3] Moscow MV Lomonosov State Univ, Belozersky Res Inst Phys Chem Biol, Moscow 119234, Russia

[4] All Russian State Ctr Qual & Standardizat Med Anim, Moscow 123022, Russia

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2024年 / 79卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

tylosin; antibiotic; fluorescence polarization; homogeneous immunoassay; antigen-fluorophore conjugate; production of antisera; food safety; ANTIBIOTIC USE; TILMICOSIN; RESIDUES; ANTIBODY; VALIDATION; HAPTEN; MILK;

D O I：

10.1134/S1061934824020096

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fluorescence polarization immunoassay method has been developed for the determination of antibiotic tylosin in honey. For this purpose, rabbits were immunized with a conjugate of tylosin and bovine serum albumin, and antisera were obtained. The conditions for the interaction of antisera in a competitive format with tylosin in the sample and with tylosin labeled with fluorescein were optimized. The developed method is characterized by a limit of detection for tylosin of 34.7 ng/mL and an analytical range of 65.2 to 564.0 ng/mL. High reproducibility of measurements using a portable detector was demonstrated, with relative standard deviation (RSD) values of 1.5-2.5%. The method was validated using honey samples, showing recovery ranging from 98 to 103.8%. An analysis time, excluding sample preparation, is 10 min.

引用

页码：154 / 161

页数：8

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