Ras signalling on the endoplasmic reticulum and the Golgi

被引：0
作者：
Vi K. Chiu
Trever Bivona
Angela Hach
J. Bernard Sajous
Joseph Silletti
Heidi Wiener
Ronald L. Johnson
Adrienne D. Cox
Mark R. Philips
机构：
[1] Cell Biology and Pharmacology,Departments of Medicine
[2] New York University School of Medicine,Departments of Radiation Oncology and Pharmacology
[3] University of North Carolina School of Medicine,undefined
来源：
Nature Cell Biology | 2002年 / 4卷
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摘要：
Current models evoke the plasma membrane (PM) as the exclusive platform from which Ras regulates signalling. We developed a fluorescent probe that reports where and when Ras is activated in living cells. We show that oncogenic H-Ras and N-Ras engage Raf-1 on the Golgi and that endogenous Ras and unpalmitoylated H-Ras are activated in response to mitogens on the Golgi and endoplasmic reticulum (ER), respectively. We also demonstrate that H-Ras that is restricted to the ER can activate the Erk pathway and transform fibroblasts, and that Ras localized on different membrane compartments differentially engages various signalling pathways. Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane.
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页码：343 / 350
页数：7
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