EBER2 RNA-induced transcriptome changes identify cellular processes likely targeted during Epstein Barr virus infection

被引：16

作者：

Eilebrecht S. ^{[1
]}

Pellay F.-X. ^{[2
]}

Odenwälder P. ^{[1
]}

Brysbaert G. ^{[2
]}

Benecke B.-J. ^{[1
]}

Benecke A. ^{[2
]}

机构：

[1] Department of Biochemistry, Ruhr University Bochum, 44780 Bochum

[2] Institut des Hautes Études Scientifiques, CNRS USR3078, Université Lille1, 91440 Bures sur Yvette, 35, route de Chartres

来源：

BMC Research Notes | / 1卷 / 1期

关键词：

Green Fluorescent Protein; Green Fluorescent Protein Positive Cell; EBER2 Expression; Bonferroni Multiple Testing Correction; Cellular Transcriptome;

D O I：

10.1186/1756-0500-1-100

中图分类号：

学科分类号：

摘要：

Background: Little is known about the physiological role of the EBER1 and 2 nuclear RNAs during Epstein Barr viral infection. The EBERs are transcribed by cellular RNA Polymerase III and their strong expression results in 10 6 to 107 copies per EBV infected cell, making them reliable diagnostic markers for the presence of EBV. Although the functions of most of the proteins targeted by EBER RNAs have been studied, the role of EBERs themselves still remains elusive. Findings: The cellular transcription response to EBER2 expression using the wild-type and an internal deletion mutant was determined. Significant changes in gene expression patterns were observed. A functional meta-analysis of the regulated genes points to inhibition of stress and immune responses, as well as activation of cellular growth and cytoskeletal reorganization as potential targets for EBER2 RNA. Different functions can be assigned to different parts of the RNA. Conclusion: These results provide new avenues to the understanding of EBER2 and EBV biology, and set the grounds for a more in depth functional analysis of EBER2 using transcriptome activity measurements. © 2008 Benecke et al; licensee BioMed Central Ltd.

引用

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