The PII protein GlnK is a pleiotropic regulator for morphological differentiation and secondary metabolism in Streptomyces coelicolor

被引：0
作者：
Eva Waldvogel
Alexander Herbig
Florian Battke
Rafat Amin
Merle Nentwich
Kay Nieselt
Trond E. Ellingsen
Alexander Wentzel
David A. Hodgson
Wolfgang Wohlleben
Yvonne Mast
机构：
[1] University of Tübingen,Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, Faculty of Science
[2] University of Tübingen,Center for Bioinformatics Tübingen, Faculty of Science
[3] SINTEF Materials and Chemistry,Department of Biotechnology
[4] University of Warwick,Department of Biological Sciences
来源：
Applied Microbiology and Biotechnology | 2011年 / 92卷
关键词：
Actinomycetes; P; protein; GlnK; Transcriptome; Glutamate;
D O I：
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学科分类号：
摘要：
GlnK is an important nitrogen sensor protein in Streptomyces coelicolor. Deletion of glnK results in a medium-dependent failure of aerial mycelium and spore formation and loss of antibiotic production. Thus, GlnK is not only a regulator of nitrogen metabolism but also of morphological differentiation and secondary metabolite production. Through a comparative transcriptomic approach between the S. coelicolor wild-type and a S. coelicolor glnK mutant strain, 142 genes were identified that are differentially regulated in both strains. Among these are genes of the ram and rag operon, which are involved in S. coelicolor morphogenesis, as well as genes involved in gas vesicle biosynthesis and ectoine biosynthesis. Surprisingly, no relevant nitrogen genes were found to be differentially regulated, revealing that GlnK is not an important nitrogen sensor under the tested conditions.
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页码：1219 / 1236
页数：17
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