Effect of Optogenetic Stimulus on the Proliferation and Cell Cycle Progression of Neural Stem Cells

被引：0
|
作者：
Shao Jun Wang
Chuan Huang Weng
Hai Wei Xu
Cong Jian Zhao
Zheng Qin Yin
机构：
[1] Third Military Medical University,Southwest Eye Hospital/Southwest Hospital, Key Laboratory of Visual Damage and Regeneration and Restoration of Chongqing
来源：
The Journal of Membrane Biology | 2014年 / 247卷
关键词：
Optogenetics; Neural stem cell; Proliferation; Cell cycle;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Modulation of stem cell proliferation is a crucial aspect of neural developmental biology and regenerative medicine. To investigate the effect of optical stimulation on neural stem cell proliferation, cells transduced with channelrhodopsin-2 (ChR2) were used to analyze changes in cell proliferation and cell cycle distribution after light stimulation. Blue light significantly inhibited cell proliferation and affected the cell cycle, which increased the percentage of cells in G1 phase and reduced the percentage in S phase. It is likely that the influence of blue light on cell proliferation and the cell cycle was mediated by membrane depolarization, which induced accumulation of p21 and p27 proteins. Our data provide additional specific evidence that membrane depolarization may inhibit neural stem cell proliferation.
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页码：493 / 500
页数：7
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