Laser phase plate for transmission electron microscopy

被引：0
作者：
Osip Schwartz
Jeremy J. Axelrod
Sara L. Campbell
Carter Turnbaugh
Robert M. Glaeser
Holger Müller
机构：
[1] University of California–Berkeley,Department of Physics
[2] Lawrence Berkeley National Laboratory,Department of Molecular and Cell Biology
[3] University of California–Berkeley,undefined
来源：
Nature Methods | 2019年 / 16卷
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摘要：
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Here, we demonstrate electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. We demonstrate the laser phase plate by imaging an amorphous carbon film. The laser phase plate provides a stable and tunable phase shift without electrostatic charging or unwanted electron scattering. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells.
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页码：1016 / 1020
页数：4
相关论文
共 52 条
  • [1] Cheng Y(2017)How Cryo-EM became so hot Cell 171 1229-1231
  • [2] Glaeser RM(2016)Visualizing the molecular sociology at the HeLa cell nuclear periphery Science 351 969-972
  • [3] Nogales E(2013)Invited Review article: methods for imaging weak-phase objects in electron microscopy Rev. Sci. Instrum. 84 111101-698
  • [4] Mahamid J(1942)Phase contrast, a new method for the microscopic observation of transparent objects Physica 9 686-193
  • [5] Glaeser RM(1995)The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules Q. Rev. Biophys. 28 171-633
  • [6] Zernike F(1947)Über die Kontraste von Atomen im Elektronenmikroskop Z. für. Naturforsch. A 2 615-15640
  • [7] Henderson R(2014)Volta potential phase plate for in-focus phase contrast transmission electron microscopy Proc. Natl Acad. Sci. USA 111 15635-229
  • [8] Boersch H(2017)Using the Volta phase plate with defocus for cryo-EM single particle analysis eLife 6 e23006-14462
  • [9] Danev R(2016)Laser control of electron matter waves Laser Photonics Rev. 10 214-195
  • [10] Buijsse B(2010)Design of an electron microscope phase plate using a focused continuous-wave laser New J. Phys. 12 073011-143
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