Molecular cloning, expression analysis, and subcellular localization of FLOWERING LOCUS T (FT) in carrot (Daucus carota L.)

被引：0
作者：
Zongxiang Zhan
Chunsha Zhang
Hongwei Zhang
Xiaojie Li
Changlong Wen
Yi Liang
机构：
[1] Beijing Academy of Agriculture and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China),Beijing Vegetable Research Center
[2] Huazhong Agricultural University,National Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology
来源：
Molecular Breeding | 2017年 / 37卷
关键词：
Carrot; Floral transition; Quantitative RT-PCR; Subcellular localization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Flowering, an important agronomic trait for seed plants, represents the end of vegetative growth and begins to reproduce. In regulating the floral transition, FLOWERING LOCUS T (FT) encoding a mobile floral signal protein that belongs to the phosphatidylethanolamine-binding protein (PEBP) family acts as critical role. Here, an FT-like gene, DcFT, was isolated and cloned from European Daucus carota L. cultivar “Nantes-H06” (GenBank accession number KY768910), and an alignment of the DcFT protein and other FT-homolog proteins showed that it shared 88.00% similarity with CsFT from Camellia sinensis. Phylogenetic tree analysis indicated that DcFT had the closest relationship with GpFT (Gypsophila paniculata). Quantitative RT-PCR was analyzed to show that the expression pattern of DcFT in inflorescences sharply increased after 10DAA and then slowly increased reaching the maximum at 30DAA. Subcellular localization made clear that the DcFT protein was located in the nucleus and cytoplasm. Broadly speaking, DcFT is an FT-like homologous gene in carrot regulating the floral transition and could be a candidate gene for accelerating the process in carrot breeding.
引用
收藏
相关论文
共 182 条
[1]  
Abe M(2005)Fd, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex Science 309 1052-639
[2]  
Kobayashi Y(2012)The genetic basis of flowering responses to seasonal cues Nat Rev Genet 13 627-1043
[3]  
Yamamoto S(2006)CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees Science 312 1040-S31
[4]  
Daimon Y(2004)Multiple pathways in the decision to flower: enabling, promoting, and resetting Plant Cell 16 S18-37
[5]  
Yamaguchi A(1968)Internal factors of plant flowering Annu Rev Plant Physiol 19 1-391
[6]  
Ikeda Y(2010)Ornamentals Biotechnol Agric For 64 369-1607
[7]  
Ahn JH(2011)Isolation and functional characterization of the FLOWERING LOCUS T homolog, the LsFT gene, in lettuce J Plant Physiol 168 1602-533
[8]  
Miller D(2015)Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT) homolog GhFT1 from Gossypium hirsutum J Integr Plant Biol 57 522-7753
[9]  
Winter VJ(2005)A single amino acid converts a repressor to an activator of flowering Proc Natl Acad Sci U S A 102 7748-921
[10]  
Banfield MJ(2012)Proteins from the FLOWERING LOCUS T-like subclade of the PEBP family act antagonistically to regulate floral initiation in tobacco Plant J 72 908-17142
12345678910