Continuous differential impedance spectroscopy of single cells

被引：0
作者：
Daniele Malleo
J. Tanner Nevill
Luke P. Lee
Hywel Morgan
机构：
[1] University of Southampton,School of Electronics and Computer Science
[2] Oxford Gene Technology,Department of Bioengineering, Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center
[3] University of California,undefined
[4] Fluxion Biosciences,undefined
来源：
Microfluidics and Nanofluidics | 2010年 / 9卷
关键词：
Impedance spectroscopy; Single-cell analysis; Microfluidic; Microfabrication;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively).
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页码：191 / 198
页数：7
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