Genetic diversity patterns of microbial communities in a subtropical riverine ecosystem (Jiulong River, southeast China)

被引：1
作者：
Lemian Liu
Jun Yang
Yongyu Zhang
机构：
[1] Chinese Academy of Sciences,Aquatic Ecohealth Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment
[2] Graduate School of Chinese Academy of Sciences,undefined
来源：
Hydrobiologia | 2011年 / 678卷
关键词：
Plankton; Microbial community composition; DGGE; Redundancy analysis; Biomonitoring; Subtropical river;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Prokaryotic and eukaryotic microbes are key organisms in aquatic ecosystems and play pivotal roles in the biogeochemical cycles, but little is known about genetic diversity of these communities in subtropical rivers. In this study, microbial planktonic communities were determined by using denaturing gradient gel electrophoresis (DGGE) analysis from the Jiulong River, southeast China, and their relationships with local environmental factors were studied. The Betaproteobacteria (26%) and Dinophyceae (26%) were the most dominant taxa in prokaryotic and eukaryotic clones derived from DGGE bands, respectively. Further, both cluster and ordination analyses of prokaryotic and eukaryotic DGGE fingerprinting resulted in three identical groups from the 15 sites, which were closely related with the environmental factors. Partial redundancy analysis (partial RDA) revealed that agricultural pollution (phosphorus and nitrogen) and saltwater intrusion (conductivity and salinity) were the main factors impacting microbial community composition, by explaining more than two-thirds of the total variation in both prokaryotic (67.0%) and eukaryotic (70.5%) communities. Moreover, the robust and quantifiable relationship between DGGE results and environmental variables indicated that the community-level molecular fingerprinting techniques could support the physicochemical assessment of riverine water quality and ecosystem health.
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页码：113 / 125
页数：12
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