Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.)

被引:0
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作者
Y. Mano
T. Komatsuda
机构
[1] National Institute of Agrobiological Sciences,
[2] Tsukuba,undefined
[3] Ibaraki 305-8602,undefined
[4] Japan,undefined
[5] Present address: National Institute of Livestock and Grassland Science,undefined
[6] Nishinasuno,undefined
[7] Tochigi 329-2793,undefined
[8] Japan,undefined
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Albinism Hordeum vulgare Quantitative trait loci Recombinant inbred Shoot differentiation;
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摘要
Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was developed from 99 recombinant inbred lines (RILs) of 'Azumamugi' × 'Kanto Nakate Gold'. The tissue-culture traits were evaluated at the F7 and F10 generations of the RILs. The RILs showed wide and continuous variations in each of the three tissue-culture traits. Three QTLs for CG, three QTLs for SD and two QTLs for GS were detected by using composite interval mapping. A QTL for SD on chromosome 3H had a large effect, and 'Kanto Nakate Gold', which has a high differentiation ability, contributed to this QTL. The location of this QTL is identical to, or very close to, the uzu locus. We discuss the relationships between tissue-culture loci in 'Azumamugi' × 'Kanto Nakate Gold' and those in other mapping populations.
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页码:708 / 715
页数:7
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