Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa

被引：0
作者：
Hai-yu Ji
Pei Chen
Juan Yu
Ying-ying Feng
An-jun Liu
机构：
[1] Tianjin University of Science and Technology,Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology
[2] QingYunTang Biotech (Beijing) Co.,undefined
[3] Ltd.,undefined
来源：
Applied Biochemistry and Biotechnology | 2019年 / 188卷
关键词：
Polysaccharides from ; Heat treatment; Structure; Antitumor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa. GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80. Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.
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页码：481 / 490
页数：9
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