Molecular analysis and virus transmission tests place Olpidium virulentus, a vector of Mirafiori lettuce big-vein virus and tobacco stunt virus, as a distinct species rather than a strain of Olpidium brassicae

被引:32
作者
Sasaya T. [1 ,3 ]
Koganezawa H. [2 ]
机构
[1] National Agricultural Research Center for Western Region, Zentsuji
[2] Kunisada Plant Breeding Station, Kaneko Seeds, Gunma
[3] Policy Planning and Evaluation Division, Minister's Secretariat, MAFF, Tokyo
关键词
Olpidium brassicae; Olpidium virulentus; rDNA-ITS; Taxonomy;
D O I
10.1007/s10327-005-0239-7
中图分类号
学科分类号
摘要
The rDNA-ITS sequences of ten single-sporangium isolates of Olpidium virulentus (a noncrucifer strain of Olpidium brassicae), which transmits Mirafiori lettuce big-vein virus (MLBVV) and tobacco stunt virus (TStV), were compared with those of six single-sporangium isolates of O. brassicae. The sequence similarity within isolates of O. virulentus or O. brassicae was almost identical (98.5%-100.0%), but was low between the two species (79.7%-81.8%). In a phylogenetic analysis of the rDNA-ITS region, O. virulentus and O. brassicae fell into two distinct clusters, indicating that O. virulentus, a vector of MLBVV and TStV, is a distinct species rather than a strain of O. brassicae. © The Phytopathological Society of Japan and Springer-Verlag 2006.
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页码:20 / 25
页数:5
相关论文
共 14 条
[1]  
Bruns T.D., White T.J., Taylor J.W., Fungal molecular systematics, Annu Rev Ecol Syst, 22, pp. 525-564, (1991)
[2]  
Campbell R.N., Fungal transmission of plant viruses, Annu Rev Phytopathol, 34, pp. 87-108, (1996)
[3]  
Koganezawa H., Takayama T., Sasaya T., Difference in resting-spore formation between crucifer and noncrucifer strains of Olpidium brassicae sensu lato (in Japanese), Jpn J Phytopathol, 70, pp. 307-313, (2004)
[4]  
Koganezawa H., Inoue H., Sasaya T., Host specificity and multiplication of eight isolates of Olpidium brassicae sensu lato and its related Olpidium sp, Bull Natl Agric Res Cent West Reg, 4, pp. 39-59, (2005)
[5]  
Kuwata S., Kubo S., Tobacco Stunt Virus. CMI/AAB Descriptions of Plant Viruses, 313, (1986)
[6]  
Lot H., Campbell R.N., Souche S., Milne R.G., Roggero P., Transmission by Olpidium brassicae of Mirafiori lettuce virus and Lettuce big-vein virus, and their roles in lettuce big-vein etiology, Phytopathology, 92, pp. 288-293, (2002)
[7]  
Rochon D., Kakani K., Robbins M., Reade R., Molecular aspects of plant virus transmission by Olpidium and Plasmodiophorid vectors, Annu Rev Phytopathol, 42, pp. 211-241, (2004)
[8]  
Sahtiyanci S., Studien über einige wurzelparasitäre Olpidiaceen, Arch Mikrobiol, 41, pp. 187-228, (1962)
[9]  
Sasaya T., Ishikawa K., Kuwata S., Koganezawa H., Molecular analysis of coat protein coding region of tobacco stunt virus shows that it is a strain of Lettuce big-vein virus in the genus Varicosavirus, Arch Virol, 150, pp. 1013-1021, (2005)
[10]  
Teakle D.S., Hiruki C., Vector specificity in Olpidium, Virology, 24, pp. 539-544, (1964)