Polyacrylamide-based glycoconjugates as tools in glycobiology

被引：0

作者：

Nicolai V Bovin

机构：

[1] Shemyakin Institute of Bioorganic Chemistry,Russian Academy of Sciences, ul

来源：

Glycoconjugate Journal | 1998年 / 15卷

关键词：

antibodies; assays; carbohydrates; cytochemistry; diagnostics; glycoconjugates; glycosyltransferases; histochemistry; immunoassay; lectins; polyacrylamide; Atri, GalNAcα1-3(Fucα1-2)Galβ; biotbiotin; BSA, bovine serum albumin; Bdi, Galα1-3Galβ; Btri, Galα1-3(Fucα1-2) Galβ; DMF, dimethylformamide; DMG, 3,6-di-O-methyl-β-D-glucopyranose; DMSO, dimethylsulphoxide; ELISA, enzyme-linked immunosorbent assay; flu, fluorescein; αGal-T, α-galactosyltransferase; Glyc, glycosyl- or glycosyl-spacer residue (mono oroligosaccharide); Mr, relative molecular weight; NC, nitrocellulose; p4NP, poly(4-nitrophenylacrylate); PA, polyacrylic acid; PAA, polyacrylamide; PAAe, poly(N-hydroxyethylacrylamide); PE, phosphatidylethanolamine; SERS, surface enhanced Raman scattering; SiaLea,Neu5Acα2-3Galβ1-3(Fucα1-4)GlcNAcβ; SiaLex,Neu5-Acα2-3Galβ1-4(Fucα1-3)GlcNAcβ;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This review describes the synthesis, physicochemical characteristics and application for studying carbohydrate-binding proteins of polyacrylamide (PAA) type neoglycoconjugates. An approach to the synthesis of conjugates based on the interaction of activated polyacrylic acid with ω-aminoalkyl glycosides has been developed. Both the molecules of Glyc-PAA and the conjugates bearing various labels and effectors, as well as sorbents, and glycosurfaces can be designed using this method. Examples of the application of the conjugates as tools for the study of lectins, antibodies, and glycosyltransferases in glycobiology, cytochemistry and histochemistry are described along with the prospects of the further development of the presented approach in glycotechnology and medicine.

引用

页码：431 / 446

页数：15

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