Production of rosmarinic acid byLavandula vera MM cell suspension in bioreactor: effect of dissolved oxygen concentration and agitation

被引:0
作者
Atanas I. Pavlov
Milen I. Georgiev
Mladenka P. Ilieva
机构
[1] Bulgarian Academy of Sciences,Department of Microbial Biosynthesis and Biotechnologies – Laboratory in Plovdiv, Institute of Microbiology
来源
World Journal of Microbiology and Biotechnology | 2005年 / 21卷
关键词
Agitation; bioreactor; dissolved oxygen; MM; rosmarinic acid;
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学科分类号
摘要
The relationship between dissolved oxygen (DO) concentration, agitation rate and growth of Lavandula vera MM and rosmarinic acid biosynthesis was investigated in 3 l laboratory bioreactor. Lavandula vera MM cell suspension accumulated the highest amounts of biomass (34.8 g/l) and rosmarinic acid (1870.6 mg/l) on day 12 of cultivation at 50% dissolved oxygen and agitation speed 100 rpm and at 30% dissolved oxygen and agitation speed 300 rpm, respectively.
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页码:389 / 392
页数:3
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  • [1] Godoy-Hernandez G.C.(2000)The exposure to Biotechnology Letters 22 921-925
  • [2] Vazquez-Flota F.A.(2002)-cinnamic acid of osmotically stressed Enzyme and Microbial Technology 30 779-791
  • [3] Loyola-Vargas V.M.(1997) cells cultures in 14-l bioreactor increases alkaloid accumulation Applied Microbiology and Biotechnology 47 683-688
  • [4] Huang S-Y.(1997)Development of a bioreactor operation strategy for L-DOPA production using Journal of Biotechnology 59 39-52
  • [5] Shen Y-W.(1965) suspension culture Physiology Plantarum 18 100-127
  • [6] Chan H-S.(1995)Rosmarinic acid production by Fresenius Journal of Analytical Chemistry 351 311-314
  • [7] Ilieva M.(2002) MM cell-suspension culture Biotechnology Letters 24 561-565
  • [8] Pavlov A.(1985)Plant cell suspension cultures: some engineering considerations Drugs of future 10 756-757
  • [9] Kieran P.M.(2000)Organic growth factor requirements of tobacco tissue cultures Biotechnology Progress 16 668-670
  • [10] MacLoughlin P.F.(1999)Spectrophotometric determination of rosmarinic acid in plant cell cultures by complexation with Fe Enzyme and Microbial Technology 24 687-693