A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopy

被引：0

作者：

Shijie Fu

Xiwen Wang

Ting Wang

Zhiping Li

Deming Han

Chunsheng Yu

Cui Yang

Han Qu

Hang Chi

Yutian Wang

Song Li

Baihui Tian

Wenliang Li

Zhiping Xia

机构：

[1] Changchun University of Science and Technology,School of Life Science and Technology

[2] Institute for Drug and Instrument Control of Joint Logistics Army,Key Laboratory of Jinlin Province for Zoonosis Prevention and Control

[3] PLA,Department of the Gastrointestinal Surgery

[4] Institute of Military Veterinary,Institute of Military Veterinary Medicine

[5] Academy of Military Medical Science,School of Pharmacy

[6] The First Hospital of Jilin University,undefined

[7] Academy of Military Medical Sciences,undefined

[8] Technology & Testing Laboratory of Lanshe Diatom New Materials Co.,undefined

[9] Ltd,undefined

[10] Jilin Medical University,undefined

来源：

Brazilian Journal of Microbiology | 2020年 / 51卷

关键词：

SERS; Antibiotics; Antibiotic susceptibility; MIC;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This study aims to develop a rapid bacterial antibiotic susceptibility test (AST) method by Bacteria-aptamer@AgNPs–surface enhanced Raman spectroscopy (SERS) and further evaluate the influence of different antibiotics on the Raman intensity of bacteria. The Raman intensity of Escherichia coli O157:H7 (E. coli O157:H7) and Staphylococcus aureus (S. aureus) in the presence of different concentrations of antibiotics in 2 h was detected by Bacteria-aptamer@AgNPs-SERS in this study. Our results found that the bacteria Raman signal peak at 735 cm−1 and the minimum inhibitory concentration (MIC) value was determined in 1 h according to Raman signals. In 2 h, the bacteria Raman signal growth at sub-MIC concentrations of four different kinds of antibiotics and the bacteria colony-forming unit (CFU) have similar enhancements. SERS utilizes special functions of rough metal surfaces and offers a huge enhancement of Raman intensities with reduced fluorescence backgrounds, which makes it an ultrasensitive tool of detection. This rapid AST method and the enhancement effect should be of value in search of new antibiotic drugs.

引用

页码：875 / 881

页数：6

共 106 条

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