Distribution of fluorocarbon quencher among micelles via pyrene fluorescence probe method

Fluorescence-quenching of pyrene in micellar system has been investigated using 1,1,2,2-tetrahydroheptadecafluorodecylpyridinium chloride (HFDePC). The new fluorocarbon quencher has a similar quenching ability as hexadecylpyridinium chloride (CPC) towards pyrene in hydrocarbon micelles if only a quencher molecule is solubilized in a micelle. The fluorocarbon quencher randomly distributed among micelles if the average occupancy number of probes per a micelle was small enough. The fluorescence behavior of pyrene was examined for hexadecyltrimethylammonium chloride (CTAC) and HFDePC mixtures. The variation of fluorescence intensity gave second cmc, reflecting the micellar immiscibility of fluorocarbon and hydrocarbon surfactants. The second cmc can be simulated by material balances of both surfactants supposing the coexistence of two kinds of mixed micelles. The fluorescence-quenching behavior suggested the enhanced micellar immiscibility probably due to nonrandom distribution of fluorocarbon quenchers among micelles.