Characterization of an Extracellular Serine Protease Gene from the Nematophagous Fungus Lecanicillium psalliotae

被引：0
作者：
Jinkui Yang
Xiaowei Huang
Baoyu Tian
Hui Sun
Junxin Duan
Wenping Wu
Keqin Zhang
机构：
[1] Yunnan University,Laboratory for Conservation and Utilization of Bio
[2] Research and Development Center of Novozymes in China,resources
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
gene cloning; serine protease; similarity comparison;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The gene encoding a cuticle-degrading serine protease was cloned from three isolates of Lecanicillium psalliotae (syn. Verticillium psalliotae) by 3′ and 5′ RACE (rapid amplification of cDNA ends) method. The gene encodes for 382 amino acids and the protein shares conserved motifs with subtilisin N and peptidase S8. Comparison of translated cDNA sequences of three isolates revealed one amino acid polymorphism at position 230. The deduced protease sequence shared high degree of similarities to other cuticle-degrading proteases from other nematophagous fungi.
引用
收藏
页码：1329 / 1334
页数：5
相关论文
共 70 条
[11]  
Garcia-Sanchez A(1992)Serine proteases structure and mechanism of catalysis Eur. J. Biochem. 204 991-1001
[12]  
Cerrato R(1994)Purification and properties of extracellular proteases produced by the nematophagous fungus Microbiology 140 2715-2723
[13]  
Larrasa J(2002)Collagenase production by nematode-trapping fungi Fungal Genet. Biol. 35 67-78
[14]  
Ambrose NC(1994)Molecular cloning and regulatory analysis of the cuticle-degrading protease structural gene from the entomopathogenic fungus Microbiology 140 1687-1695
[15]  
Parra A(1996)The nematophagous fungus FEMS Microbiol. Lett. 145 261-265
[16]  
Alonso JM(2004) produces a chymoelastase-like protease which hydrolyses host nematode proteins Mycologia 96 16-22
[17]  
Hermoso-de-Mendoza M(undefined)Purification and characterization of chitinases from the nematophagous fungi undefined undefined undefined-undefined
[18]  
Rey JM(undefined) and undefined undefined undefined-undefined
[19]  
Mine MO(undefined)Purification and characterization of an extracellular serine protease from the nematode-trapping fungus undefined undefined undefined-undefined
[20]  
Carnegie PR(undefined)A method for the large scale isolation of high transformation efficiency fungal genomic DNA undefined undefined undefined-undefined
1234567