Characterization of an Extracellular Serine Protease Gene from the Nematophagous Fungus Lecanicillium psalliotae

被引：0
作者：
Jinkui Yang
Xiaowei Huang
Baoyu Tian
Hui Sun
Junxin Duan
Wenping Wu
Keqin Zhang
机构：
[1] Yunnan University,Laboratory for Conservation and Utilization of Bio
[2] Research and Development Center of Novozymes in China,resources
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
gene cloning; serine protease; similarity comparison;
D O I：
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中图分类号：
学科分类号：
摘要：
The gene encoding a cuticle-degrading serine protease was cloned from three isolates of Lecanicillium psalliotae (syn. Verticillium psalliotae) by 3′ and 5′ RACE (rapid amplification of cDNA ends) method. The gene encodes for 382 amino acids and the protein shares conserved motifs with subtilisin N and peptidase S8. Comparison of translated cDNA sequences of three isolates revealed one amino acid polymorphism at position 230. The deduced protease sequence shared high degree of similarities to other cuticle-degrading proteases from other nematophagous fungi.
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页码：1329 / 1334
页数：5
相关论文
共 70 条
[1]  
Åhman J(1996)Sequence analysis and regulation of a gene encoding a cuticle-degrading serine protease from the nematophagous fungus Microbiology 142 1605-1616
[2]  
EK B(1995)A basic serine protease from Microbiology 141 775-784
[3]  
Rask L(2004) with biological activity against FEMS Microbiol. Lett. 231 53-57
[4]  
Tunlid A(1997) eggs Protein Eng. 10 1-6
[5]  
Bonants PJ(1991)Characterization of an extracellular serine protease gene (nasp gene) from Agric. Biol. Chem. 55 471-477
[6]  
Fitters PF(1995)Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites FEMS Microbiol. Lett. 125 211-218
[7]  
Thijs H(2003)Cloning and nucleotide sequences of the complementary and genomic DNA for the alkaline protease from FEMS Immunol. Med. Microbiol. 36 27-32
[8]  
Den BE(1977)Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Annu. Rev. Biochem. 46 331-358
[9]  
Waalwijk C(1990)Detection of gene mutations related with drug resistance in Can. J. Microbiol. 36 530-537
[10]  
Henfling JW(1980) from leprosy patients using Touch-Down (TD) PCR Appl. Environ. Microbiol. 40 567-570
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