Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines

被引:0
作者
Chun-qiao Xiao
Ru-an Chi
Huan He
Wen-xue Zhang
机构
[1] Central South University,Key Laboratory of Biometallurgy of Ministry of Education
[2] Wuhan Institute of Technology,Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology
[3] Yunnan Phosphate Chemical Group Co. Ltd,undefined
来源
Journal of Central South University of Technology | 2009年 / 16卷
关键词
tricalcium phosphate (TCP); (; ); phosphate mines; phosphorus (P); gluconic acid;
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中图分类号
学科分类号
摘要
The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines (Hubei, China), were studied in National Botanical Research Institute’s phosphate (NBRIP) growth medium containing tricalcium phosphate (TCP) as sole phosphorus (P) source. The strain YC is identified as Stenotrophomonas maltophilia (S. maltophilia) based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis. It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source, respectively, in NBRIP growth medium.
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页码:581 / 587
页数:6
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