Physiological and phylogenetic variability of Mexican Metarhizium strains

被引：0
作者：
Carolina Brunner-Mendoza
Soumya Moonjely
María del Rocío Reyes-Montes
Conchita Toriello
Michael Bidochka
机构：
[1] Universidad Nacional Autónoma de México,Departamento de Microbiología y Parasitología, Facultad de Medicina
[2] Brock University,Department of Biological Sciences
来源：
BioControl | 2017年 / 62卷
关键词：
Hypocreales; Clavicipitaceae; Virulence; RFLP; 5′TEF; Pr1;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Metarhizium Sorokin is a wide spread entomopathogenic fungal genus that is also a root symbiont and some isolates may be potentially useful in agriculture applications. Physiological variations applicable in biological control have not been fully evaluated in Mexican strains. This study identified 30 strains from different insect hosts and states of Mexico selected because they represent the main sugar cane and corn plantations of the country. Their thermotolerance profiles (8, 15, 28 and 35 °C), insect virulence (LT50 in Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) larvae), possible association with plant roots, and phylogenetic placement (using 5′TEF, IGS sequence information, and RFLP analysis) were assessed. Results showed no cold-active strains and most showed restricted growth at 35 °C. Insect virulence bioassays against G. mellonella showed highly virulent strains. Most of the strains showed an ability to associate with roots of Phaseolus vulgaris (Burkart) (Fabales: Fabaceae). RFLP analysis with 5′TEF and Pr1 revealed three distinct genetic groups. Phylogenetic analysis identified the 30 strains as Metarhizium anisopliae. (Metchnikoff) Sorokin, M. robertsii (J.F. Bisch., Rehner & Humber), M. guizhouense (Q. T. Chen & H. L. Guo), or M. pinghaense (Q.T. Chen & H.L. Guo), as well as two strains that belong to a new clade (Metarhizium sp. indet. 1). These data demonstrate the complexity of Metarhizium species and the need to study their physiological and phylogenetic aspects for a more adequate assessment of their potential use in agricultural systems.
引用
收藏
页码：779 / 791
页数：12
相关论文
共 130 条
  • [11] Bischoff JF(2011)Characterization and phylogeny of J Invertebr Pathol 106 289-295
  • [12] Rehner SA(2016) spp. strains (Ascomycota: Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-α sequences Parasit Vectors 9 176-187
  • [13] Humber RA(1994)Effect of constant temperatures on germination, radial growth and virulence of Ann Rev Entomol 39 293-322
  • [14] Bruck DJ(2003) to three species of African tephritid fruit flies FEMS Microbiol Lett 229 127-132
  • [15] Carrillo-Benítez MG(2016)A taxonomic revision of Neotrop Entomol 45 80-87
  • [16] Guzmán-Franco AW(2002) based on a phylogenetic analysis of rDNA sequence data App Environ Microbiol 68 6383-6387
  • [17] Alatorre-Rosas R(2001)Influence de la temperature sur la croissance in vitro d′hyphomycetes entomopathogens Bioinformatics 17 754-755
  • [18] Enríquez-Vara JN(2013)Diversity of rhizosphere associated entomopathogenic fungi of perennial herbs, shrubs and coniferos trees Mol Ecol Resour 13 210-217
  • [19] D’Alessandro CP(2014)Survival and immune response of the Chagas vector Mycologia 106 811-829
  • [20] Jones LR(1997) (Hemiptera: Reduviidae) against two entomopathogenic fungi, Mycol Res 101 257-265
12345678910