Involvement of virulence properties and antibiotic resistance in Escherichia coli strains causing pyelonephritis in children

被引:0
作者
Ján Koreň
Katarína Čurová
Marta Kmeťová
Leonard Siegfried
Viktor Jankó
László Kovács
Helena Hupková
Ján Luha
机构
[1] Comenius University and University Hospital Bratislava,Institute of Microbiology, Faculty of Medicine
[2] Pavol Jozef Šafárik University and Louis Pasteur University Hospital,Institute of Medical Microbiology and Clinical Microbiology, Faculty of Medicine
[3] Comenius University Medical School,2nd Department of Pediatrics
[4] University Children’s Hospital,Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine
[5] Comenius University and University Hospital Bratislava,undefined
来源
Folia Microbiologica | 2013年 / 58卷
关键词
Antimicrobial Resistance; Cefuroxime; Pyelonephritis; AmpC; Acute Pyelonephritis;
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摘要
A total of 145 Escherichia coli strains causing pyelonephritis in children were investigated for the prevalence of genes encoding the following virulence factors (VFs): P fimbria (67.6 %), S fimbria (53.8 %), AFA adhesins (2.8 %), cytotoxic necrotizing factor 1 (37.9 %), α-hemolysin (41.4 %), and aerobactin (71.7 %). One hundred and thirty-six (93.8 %) isolates harbored at least one of the virulence genes detected in the present study. Statistically significant co-occurrent presence of two VF genes was found for α-hly–cnf1, α-hly–sfa, cnf1–sfa (p < 0.001), and α-hly–pap (p = 0.001). Twenty-six profiles of VF genes were detected in this study. The combinations of aer–pap and aer–pap–sfa–α-hly–cnf1 were presented with the highest frequency—both of them in 28 isolates (19.3 %). All E. coli strains included in the study were susceptible to meropenem, amikacin, and tobramycin; the highest frequency resistance was found toward ampicillin (43.4 %), piperacillin (31.7 %), tetracycline (15.9 %), and trimethoprim/sulfamethoxazole (11.7 %). The resistance to the other tested antimicrobial drugs did not exceed 3 % incidence. Overall, 55.9 % strains were susceptible to all tested anti-infective agents. Antimicrobial resistance of E. coli strains toward trimethoprim/sulfamethoxazole statistically significantly correlated with the presence of α-hly (p < 0.001), sfa (p < 0.01), and cnf1 (p < 0.05).
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页码:53 / 59
页数:6
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