Evaluation of tumor necrosis factor-alpha (TNF-α); gamma interferon (IFN-γ) genes and oxidative stress in sheep: immunological responses induced by Oestrus ovis (Diptera: Oestridae) infestation

被引：27
作者：
Attia M.M. [1 ]
El-Gameel S.M. [1 ]
Ismael E. [2 ]
机构：
[1] Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Giza
[2] Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza
来源：
Journal of Parasitic Diseases | 2020年 / 44卷 / 2期
关键词：
Gamma interferon (IFN-γ); Oestrus ovis; Oxidative stress in sheep; Sheep nasal fly; Tumor necrosis factor alpha (TNFα);
D O I：
10.1007/s12639-020-01220-w
中图分类号：
学科分类号：
摘要：
This study aimed to evaluate the cell mediated immune responses against Oestrus ovis (O. ovis) in sheep through measurement of the changes in mRNA expression of the tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) cytokines using quantitative Real time-PCR (qRt-PCR). Also; to detect the role of Oestrus ovis infestation in the oxidative stress markers in sheep. Fifty sheep head were examined in Cairo abattoir from the period of May to August 2019. Sera were separated and collected for measurement of nitric oxide, zinc and malondialdehyde (MDA). While TNF-α and IFN-γ mRNA were extracted from nasal mucosa. Levels of IFN-γ and TNF-α were significantly higher in infested sheep than that in non-infested one. Also, oxidative stresses were indicated by high level of nitric oxide as one of reactive oxygen species (ROS) and serum MDA as oxidative stress marker and low antioxidant capacity (zinc concentration in serum) in infested sheep. The obtained results indicated that measurements of TNF-α and IFN-γ cytokines using qRT-PCR could be used as an association and reproducible quantitative method for the diagnosis of O. ovis infestation in sheep. © 2020, Indian Society for Parasitology.
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页码：332 / 337
页数：5
相关论文
共 42 条
[11]  
Caracappa S., Rilli S., Zanghi P., Di Marco V., Dorchies P., Epidemiology of ovine oestrosis (Oestrus ovis Linné 1761, Diptera: Oestridae) in Sicily, Vet Parasitol, 92, pp. 233-237, (2000)
[12]  
Channappanavar R., Singh K.P., Singh R., Umeshappa C.S., Ingale S.L., Pandey A.B., Enhanced proinflammatory cytokine activity during experimental bluetongue virus-1 infection in Indian native sheep, Vet Immunol Immunopathol, 145, 1-2, pp. 485-492, (2012)
[13]  
Coussens P.M., Verman N., Coussens M.A., Elftman M.D., McNulty A.M., Cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with mycobacterium avium subsp. paratuberculosis: evidence for an inherent proinflammatory gene expression pattern, Infect Immun, 72, 3, pp. 1409-1422, (2004)
[14]  
Donahoe S.L., Phalen D.N., McAllan B.M., O'Meally D., McAllister M.M., Ellis J., Slapeta J., Differential gamma interferon- and tumor necrosis factor alpha-driven cytokine response distinguishes acute infection of a metatherian host with Toxoplasma gondii and Neospora caninum, Infect Immun, 85, 6, (2017)
[15]  
Dubey J.P., Schares G., Neosporosis in animals—the last five years, Vet Parasitol, 180, pp. 90-108, (2011)
[16]  
El-Tahawy A.S., The prevalence of selected diseases and syndromes affecting Barki sheep with special emphasis on their economic impact, Small Rumin Res, 90, pp. 83-87, (2010)
[17]  
Gaaboub L.A., The distribution and seasonal dynamics of Oestrus ovis Linne infesting nasal cavities and sinuses of sheep in Egypt, Vet. Parasiotl, 4, 1, pp. 79-82, (1978)
[18]  
Giulietti A., Overbergh L., Valckx D., Decallonne B., Bouillon R., Mathieu C., An overview of real-time quantitative PCR: applications to quantify cytokine gene expression, Methods, 25, 4, pp. 386-401, (2001)
[19]  
Hassanin M.A., Abdel-Gawad A.M., Ramadan E.I., Incidence, monthly prevalence and bionomics of Oestrus ovis infesting sheep in Egypt, J Egypt Vet Med Assoc, 49, pp. 431-443, (1989)
[20]  
Heidarpour M., Mohri M., Borji H., Moghdass E., Oxidant/antioxidant status in cattle with liver cystic echinococcosis, Vet Parasitol, 195, 1-2, pp. 131-135, (2013)
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