Coconut oil extract mitigates testicular injury following adjuvant treatment with antiretroviral drugs

被引：23

作者：

Ogedengbe O.O. ^{[1
,2
]}

Jegede A.I. ^{[1
,3
]}

Onanuga I.O. ^{[1
,4
]}

Offor U. ^{[1
]}

Naidu E.C.S. ^{[1
]}

Peter A.I. ^{[1
,5
]}

Azu O.O. ^{[1
]}

机构：

[1] Discipline of Clinical Anatomy, Nelson R. Mandela School of Medicine, University of Kwazulu-Natal, Durban

[2] Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti

[3] Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso

[4] Department of Anatomy, Faculty of Biomedical Sciences, Kampala International University, Western Campus, Ishaka

[5] Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo-Nigeria, Uyo

来源：

Toxicological Research | 2016年 / 32卷 / 4期

关键词：

Andrology; Antiretroviral therapy; Coconut oil; Histomorphology; Stains; Testis;

D O I：

10.5487/TR.2016.32.4.317

中图分类号：

学科分类号：

摘要：

Increased access to highly active antiretroviral therapy (HAART) has made the management of drug toxicities an increasingly crucial component of HIV. This study investigated the effects of adjuvant use of coconut oil and HAART on testicular morphology and seminal parameters in Sprague- Dawley rats. Twelve adult male Sprague-Dawley rats, weighing 153~169 g were distributed into four groups (A-D) and treated as follows: A served as control (distilled water); B (HAART cocktail- Zidovudine, Lamivudine and Nevirapine); C (HAART + Virgin coconut oil 10 mL/kg) and D (Virgin coconut oil 10 mL/kg). After 56 days of treatment, animals were killed and laparotomy to exercise the epididymis for seminal fluid analyses done whilst testicular tissues were processed for histo-morphometric studies. Result showed a significant decline in sperm motility (P < 0.05) and count (P < 0.0001) in HAART-treated animals while there was insignificant changes in other parameters in groups C and D except count that was reduced (P < 0.0001) when compared with controls. Histomorphological studies showed HAART caused disorders in seminiferous tubular architecture with significant (P < 0.01) decline in epithelial height closely mirrored by extensive reticulin framework and positive PAS cells. Adjuvant Virgin coconut oil + HAART resulted in significant decrease in seminiferous tubular diameter (P < 0.05), but other morphometric and histological parameters were similar to control or Virgin coconut oil alone (which showed normal histoarchitecture levels). While derangements in testicular and seminal fluid parameters occurred following HAART, adjuvant treatment with Virgin coconut oil restored the distortions emanating thereof.

引用

页码：317 / 325

页数：8

共 54 条

[51]

Nerurkar P.V., Shikuma C.M., Nerurkar V.R., Sterol regulatory element-binding proteins and reactive oxygen species: potential role in highly-active antiretroviral therapy (HAART)-associated lipodystrophy, Clini. Biochem, 34, pp. 519-529, (2001)

[52]

Carandang E.V., Health benefits of virgin coconut oil, Indian Coconut J, 31, pp. 8-12, (2008)

[53]

Khor H., Rajendran R., Gopalakrishnan M., Tee E.S., The role of unsaponifiable components in the lipidemic property of olive oil, Malays. J. Nutr, 4, pp. 81-90, (1998)

[54]

Calvin H.I., Cooper G.W., Wallace E., Evidence that selenium in rat sperm is associated with a cysteine-rich structural protein of the mitochondrial capsules, Mol. Reprod. Dev, 4, pp. 139-149, (1981)

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