Coconut oil extract mitigates testicular injury following adjuvant treatment with antiretroviral drugs

被引：23
作者：
Ogedengbe O.O. [1 ,2 ]
Jegede A.I. [1 ,3 ]
Onanuga I.O. [1 ,4 ]
Offor U. [1 ]
Naidu E.C.S. [1 ]
Peter A.I. [1 ,5 ]
Azu O.O. [1 ]
机构：
[1] Discipline of Clinical Anatomy, Nelson R. Mandela School of Medicine, University of Kwazulu-Natal, Durban
[2] Department of Anatomy, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti
[3] Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso
[4] Department of Anatomy, Faculty of Biomedical Sciences, Kampala International University, Western Campus, Ishaka
[5] Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo-Nigeria, Uyo
来源：
Toxicological Research | 2016年 / 32卷 / 4期
关键词：
Andrology; Antiretroviral therapy; Coconut oil; Histomorphology; Stains; Testis;
D O I：
10.5487/TR.2016.32.4.317
中图分类号：
学科分类号：
摘要：
Increased access to highly active antiretroviral therapy (HAART) has made the management of drug toxicities an increasingly crucial component of HIV. This study investigated the effects of adjuvant use of coconut oil and HAART on testicular morphology and seminal parameters in Sprague- Dawley rats. Twelve adult male Sprague-Dawley rats, weighing 153~169 g were distributed into four groups (A-D) and treated as follows: A served as control (distilled water); B (HAART cocktail- Zidovudine, Lamivudine and Nevirapine); C (HAART + Virgin coconut oil 10 mL/kg) and D (Virgin coconut oil 10 mL/kg). After 56 days of treatment, animals were killed and laparotomy to exercise the epididymis for seminal fluid analyses done whilst testicular tissues were processed for histo-morphometric studies. Result showed a significant decline in sperm motility (P < 0.05) and count (P < 0.0001) in HAART-treated animals while there was insignificant changes in other parameters in groups C and D except count that was reduced (P < 0.0001) when compared with controls. Histomorphological studies showed HAART caused disorders in seminiferous tubular architecture with significant (P < 0.01) decline in epithelial height closely mirrored by extensive reticulin framework and positive PAS cells. Adjuvant Virgin coconut oil + HAART resulted in significant decrease in seminiferous tubular diameter (P < 0.05), but other morphometric and histological parameters were similar to control or Virgin coconut oil alone (which showed normal histoarchitecture levels). While derangements in testicular and seminal fluid parameters occurred following HAART, adjuvant treatment with Virgin coconut oil restored the distortions emanating thereof.
引用
收藏
页码：317 / 325
页数：8
相关论文
共 54 条
[1]  
Bachanova V., Connors J.M., Hodgkin lymphoma in the elderly, pregnant, and HIV-infected, Semin. Hematol, 53, pp. 203-208, (2016)
[2]  
Greenberg K.I., Perazella M.A., Atta M.G., HIV and HCV medications in end-stage renal disease, Semin. Dial, 28, pp. 397-403, (2015)
[3]  
Scientific Coordination Committee Marcotullio S., Andreoni M., Antinori A., d'Arminio Monforte A., Di Perri G., Galli M., Ippolito G., Perno C.F., Rizzardini G., Lazzarin A., Cinque P., Fares G., Foglia E., Gervasoni C., Murri R., Nozza S., Rusconi S., The Less Drugs Regimens (LDRs) therapy approach in HIV-1: an Italian expert panel perspective for the long-term management of HIV-1 infection, New Microbiol, 35, pp. 259-277, (2012)
[4]  
Pitt J., Myer L., Wood R., Quality of life and the impact of drug toxicities in a South African community-based antiretroviral programme, J. Int. AIDS Soc, 12, (2009)
[5]  
Nagiah S., Phulukdaree A., Chuturgoon A., Mitochondrial and oxidative stress response in HepG2 cells following acute and prolonged exposure to antiretroviral drugs, J. Cell. Biochem, 116, pp. 1939-1946, (2015)
[6]  
Casado J., Mena A., Banon S., Castro A., Quereda C., Moreno A., Pedreira J., Moreno S., Liver toxicity and risk of discontinuation in HIV/hepatitis C virus-coinfected patients receiving an etravirine-containing antiretroviral regimen: influence of liver fibrosis, HIV Med, 17, pp. 62-67, (2016)
[7]  
da Rocha I.M., Gasparotto A.S., Lazzaretti R.K., Notti R.K., Sprinz E., Mattevi V.S., Polymorphisms associated with renal adverse effects of antiretroviral therapy in a Southern Brazilian HIV cohort, Pharmacogenet. Genomics, 25, pp. 541-547, (2015)
[8]  
Dorsey J.L., Mangus L.M., Hauer P., Ebenezer G.J., Queen S.E., Laast V.A., Adams R.J., Mankowski J.L., Persistent peripheral nervous system damage in simian immunodeficiency virus-infected macaques receiving antiretroviral therapy, J. Neuropathol. Exp. Neurol, 74, pp. 1053-1060, (2015)
[9]  
Azu O.O., Naidu E.C., Naidu J.S., Masia T., Nzemande N.F., Chuturgoon A., Singh S., Testicular histomorphologic and stereological alterations following short-term treatment with highly active antiretroviral drugs (HAART) in an experimental animal model, Andrology, 2, pp. 772-779, (2014)
[10]  
Adaramoye O.A., Akanni O.O., Adewumi O.M., Owumi S.E., Lopinavir/ritonavir, an antiretroviral drug, lowers sperm quality and induces testicular oxidative damage in rats, Tokai J. Exp. Clin. Med, 40, pp. 51-57, (2015)
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