Determination of vitamin K1 in blood serum by high-performance liquid chromatography

被引：0
作者：
O. V. Sokolova
I. A. Potapova
机构：
[1] Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology,
来源：
Journal of Analytical Chemistry | 2016年 / 71卷
关键词：
vitamin K; HPLC; liquid extraction; blood serum;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A method is proposed for the determination of vitamin K1 concentration in blood serum using HPLC on a Gemini C18 (250 mm × 4.6 mm ID, 5 μm) column and elution with a methanol–acetonitrile–dichloromethane (45 : 50 : 5, v/v) mobile phase at a flow rate of 1 mL/min and detection at 248 nm. The limit of detection for the vitamin is 0.5 ng/mL and relative error is 19%. The volume of sample in the column is 100 μL. The method has been applied to the evaluation of vitamin K1 deficit in a human body in medical practice.
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页码：737 / 740
页数：3
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