Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets

被引：0
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作者：
J. M. Myers
P. W. Simon
机构：
[1] Department of Horticulture,
[2] 1575 Linden Drive,undefined
[3] University of Wisconsin,undefined
[4] Madison,undefined
[5] WI 53706,undefined
[6] USA,undefined
[7] USDA Vegetable Crops Research,undefined
[8] Department of Horticulture,undefined
[9] 1575 Linden Drive,undefined
[10] University of Wisconsin,undefined
[11] Madison,undefined
[12] WI 53706,undefined
[13] USA Fax: (608) 262-4743 E-mail: psimon@facstaff.wisc.edu,undefined
来源：
Plant Cell Reports | 1998年 / 17卷
关键词：
Key words Garlic; Allium sativum L.; Root segments; Regeneration; Picloram;
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学科分类号：
摘要：
Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μm 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μm 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μm 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
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页码：726 / 730
页数：4
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