The molecular biology of intracellular events during Coronavirus infection cycle

被引：7

作者：

Jain J. ^{[1
]}

Gaur S. ^{[1
]}

Chaudhary Y. ^{[1
]}

Kaul R. ^{[1
]}

机构：

[1] Department of Microbiology, University of Delhi, South Campus, New Delhi

来源：

VirusDisease | 2020年 / 31卷 / 2期

关键词：

Coronavirus; CoV-2; COVID-19;

D O I：

10.1007/s13337-020-00591-1

中图分类号：

学科分类号：

摘要：

CoV-2 which is the causative agent of COVID-19 belongs to genus betacoronaviruses. The sequence analysis of S protein of CoV-2 has shown that it has acquired a ‘polybasic cleavage site’ consisting of 12 aminoacids that has been predicted to enable its cleavage by other cellular proteases possibly increasing its transmissibility. The aminoacids present in receptor binding domain of S protein of SARS CoV which are critical for its binding to cellular receptor are different in CoV-2. The presence of heptanucleotide slippery sequence in ORF1 resulting in ribosomal frameshifting, and presence of transcription regulatory sequences between ORFs resulting in discontinuous transcription, are peculiar features of Coronavirus infection cycle. The exonuclease activity of nsp14 provides possible proofreading ability to RNA polymerase makes coronaviruses different from other RNA viruses allowing coronaviruses to maintain their relatively large genome size. This mini-review summarizes the peculiar features of Coronaviruses genome and the critical events during the infection cycle with focus on CoV-2. © 2020, Indian Virological Society.

引用

页码：75 / 79

页数：4

共 24 条

[1]

Andersen K.G., Rambaut A., Lipkin W.I., Holmes E.C., Garry R.F., The proximal origin of SARS-CoV-2, Nat Med, 26, 4, pp. 450-452, (2020)

[2]

Barcena M., Oostergetel G.T., Bartelink W., Faas F.G., Verkleij A., Rottier P.J., Koster A.J., Bosch B.J., Cryo-electron tomography of mouse hepatitis virus: Insights into the structure of the coronavirion, Proc Natl Acad Sci U S A, 106, pp. 582-587, (2009)

[3]

Channappanavar R., Fehr A.R., Vijay R., Mack M., Zhao J., Meyerholz D.K., Perlman S., Dysregulated Type I interferon and inflammatory monocyte-macrophage responses cause lethal pneumonia in SARS-CoV-infected mice, Cell Host Microbe, 19, pp. 181-193, (2016)

[4]

Ferron F., Subissi L., Silveira De Morais A.T., Le N.T.T., Sevajol M., Gluais L., Decroly E., Vonrhein C., Bricogne G., Canard B., Imbert I., Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA, Proc Natl Acad Sci U S A, 115, pp. E162-E171, (2018)

[5]

Fung S.Y., Yuen K.S., Ye Z.W., Chan C.P., Jin D.Y., A tug-of-war between severe acute respiratory syndrome coronavirus 2 and host antiviral defence: lessons from other pathogenic viruses, Emerg Microbes Infect, 9, pp. 558-570, (2020)

[6]

Gralinski L.E., Baric R.S., Molecular pathology of emerging coronavirus infections, J Pathol, 235, pp. 185-195, (2015)

[7]

Hagemeijer M.C., Monastyrska I., Griffith J., van der Sluijs P., Voortman J., Henegouwen P.M., Vonk A.M., Rottier P.J., Reggiori F., de Haan C.A., Membrane rearrangements mediated by coronavirus nonstructural proteins 3 and 4, Virology, 458-459, pp. 125-135, (2014)

[8]

Irigoyen N., Firth A.E., Jones J.D., Chung B.Y., Siddell S.G., Brierley I., High-resolution analysis of coronavirus gene expression by RNA sequencing and ribosome profiling, PLoS Pathog, 12, (2016)

[9]

Kamitani W., Narayanan K., Huang C., Lokugamage K., Ikegami T., Ito N., Kubo H., Makino S., Severe acute respiratory syndrome coronavirus nsp1 protein suppresses host gene expression by promoting host mRNA degradation, Proc Natl Acad Sci U S A, 103, pp. 12885-12890, (2006)

[10]

Kim I.S., Jenni S., Stanifer M.L., Roth E., Whelan S.P., van Oijen A.M., Harrison S.C., Mechanism of membrane fusion induced by vesicular stomatitis virus G protein, Proc Natl Acad Sci U S A, 114, pp. E28-E36, (2017)

← 1 2 3 →