Structure and function of seed storage proteins in faba bean (Vicia faba L.)

被引：0
作者：
Yujiao Liu
Xuexia Wu
Wanwei Hou
Ping Li
Weichao Sha
Yingying Tian
机构：
[1] Qinghai Academy of Agricultural and Forestry Science,State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University
来源：
3 Biotech | 2017年 / 7卷
关键词：
Faba bean; Protein subunit; LC–ESI–MS/MS; Bioinformatics; Structure; Function;
D O I：
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中图分类号：
学科分类号：
摘要：
The protein subunit is the most important basic unit of protein, and its study can unravel the structure and function of seed storage proteins in faba bean. In this study, we identified six specific protein subunits in Faba bean (cv. Qinghai 13) combining liquid chromatography (LC), liquid chromatography-electronic spray ionization mass (LC–ESI–MS/MS) and bio-information technology. The results suggested a diversity of seed storage proteins in faba bean, and a total of 16 proteins (four GroEL molecular chaperones and 12 plant-specific proteins) were identified from 97-, 96-, 64-, 47-, 42-, and 38-kD-specific protein subunits in faba bean based on the peptide sequence. We also analyzed the composition and abundance of the amino acids, the physicochemical characteristics, secondary structure, three-dimensional structure, transmembrane domain, and possible subcellular localization of these identified proteins in faba bean seed, and finally predicted function and structure. The three-dimensional structures were generated based on homologous modeling, and the protein function was analyzed based on the annotation from the non-redundant protein database (NR database, NCBI) and function analysis of optimal modeling. The objective of this study was to identify the seed storage proteins in faba bean and confirm the structure and function of these proteins. Our results can be useful for the study of protein nutrition and achieve breeding goals for optimal protein quality in faba bean.
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