Gold nanoparticle-labeled detection antibodies for use in an enhanced electrochemical immunoassay of hepatitis B surface antigen in human serum

被引:1
|
作者
Sheng Wu
Zhaoyang Zhong
Dong Wang
Mengxia Li
Yi Qing
Nan Dai
Zengpeng Li
机构
[1] 101th Hospital of Chinese People’s Liberation Army,Department of Hepatobiliary Surgery
[2] Third Military Medical University,Department of Cancer Center, Research Institute of Surgery, Daping Hospital
来源
Microchimica Acta | 2009年 / 166卷
关键词
Electrochemical immunosensor; Bio-barcode; Nanogold particles; Hepatitis B; Surface antigen; Sandwich-type enzyme immunoassay;
D O I
暂无
中图分类号
学科分类号
摘要
The application of gold nanoparticle-based electrochemical immunoassays have been extensively studied for the detection of hepatitis B surface antigen (HBsAg), but most often they exhibit low sensitivity. We describe the fabrication of a new electrochemical immunoassay for signal amplification of the antigen-antibody reaction combined with the nanogold-based bio-barcode technique. Hepatitis B surface antibody (HBsAb) was initially immobilized on a nanogold/thionine/DNA-modified gold electrode, and then a sandwich-type immunoassay format was employed for the detection of HBsAg using nanogold-codified horseradish peroxidase-HBsAb conjugates as secondary antibodies. Under optimal conditions, the current response of the sandwich-type immunocomplex relative to the H2O2 system was proportional to HBsAg concentration in the range from 0.5 to 650 ng·mL−1 with a detection limit of 0.1 ng·mL−1 (S/N = 3). The precision, reproducibility and stability of the immunosensor were acceptable. Subsequently, the immunosensors were used to assay HBsAg in human serum specimens. Analytical results were in agreement with those obtained by the standard chemiluminescence enzyme-linked immunosorbent assay.
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页码:269 / 275
页数:6
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