Genetic Diversity of Empoasca flavescens Fabricius (Homoptera: Cicadellidae), an Emerging Pest of Tea from Sub-Himalayan Plantations of West Bengal, India

被引：4

作者：

Saha D. ^{[1
]}

Mukhopadhyay A. ^{[1
]}

Bahadur M. ^{[2
]}

机构：

[1] Entomology Research Unit, Department of Zoology, University of North Bengal, P.O. North Bengal University, Siliguri, 734013, Darjeeling, West Bengal

[2] Genetics and Molecular Biology Laboratory, Department of Zoology, University of North Bengal, P.O. North Bengal University, Siliguri, 734013, Darjeeling, West Bengal

来源：

Proceedings of the Zoological Society | 2012年 / 65卷 / 2期

关键词：

Conventional tea plantations; Empoasca flavescens; Genetic variability; Organic tea plantations; RAPD-PCR; Tea pest population;

D O I：

10.1007/s12595-012-0031-6

中图分类号：

学科分类号：

摘要：

Empoasca flavescens Fabricius (Homoptera: Cicadellidae), is an emerging insect pest of tea, Camelia sinensis L. (Theaceae), in sub-Himalayan region of West Bengal. Use of synthetic insecticides in tea plantations against insect pests may have resulted in development of resistant E. flavescens populations. This was tested by recording genetic variations in the populations of E. flavescens collected from three different locations of North Bengal. Three populations were studied using RAPD-PCR. Polymorphism in populations was assayed using eight random primers resulting in amplification of 53 loci. The polymorphic loci recorded were 83.02, 84.92 and 88.68 % in Darjeeling hill, Terai and the Dooars populations, respectively. The genetic distance between Darjeeling hill and Terai, Darjeeling hill and the Dooars populations were found to be 0.0255 and 0.0332, respectively. UPGMA dendrogram based on the ‘Nei’s (Genetics 89: 583–590, 1978) genetic distance method’ showed greater genetic distance of Darjeeling population from Terai as compared to Dooars populations. © 2012, Zoological Society, Kolkata, India.

引用

页码：126 / 131

页数：5

共 35 条

[21]

Nei M., Estimation of average heterozygosity and genetic distances from a small number of individuals, Genetics, 89, pp. 583-590, (1978)

[22]

Nei M., Molecular evolutionary genetics, (1987)

[23]

Roy S., Gurusubramanian G., Mukhopadhyay A., Insecticide persistence and residual toxicity monitoring in tea mosquito bug Helopeltis theivora Waterhouse (Heteroptera: Hemiptera: Miridae) in Dooars, West Bengal, Resistant Pest Management Newsletter, 17, pp. 9-15, (2008)

[24]

Roy S., Mukhopadhyay A., Gurusubramanian G., Pronotal colour variation in Helopeltis theivora waterhouse (Miridae: Heteroptera) in the sub-Himalayan Dooars tea plantation of North Bengal, India, World Journal of Zoology, 4, 2, pp. 76-78, (2009)

[25]

Roy S., Mukhopadhyay A., Gurusubramanian G., Development of resistance to endosulphan in populations of the tea mosquito bug Helopeltis theivora (Heteroptera: Miridae) from organic and conventional tea plantations in India, Interenational Journal of Tropical Insect Science, 30, 2, pp. 61-66, (2010)

[26]

Saha D., Roy S., Mukhopadhyay A., Seasonal incidence and enzyme-based susceptibility to synthetic insecticides in two upcoming sucking insect pests of tea, Phytoparasitica, 40, pp. 105-115, (2012)

[27]

Sambrook J.E.F., Fritschmaniatis T., Molecular Cloning: A Laboratory Manual, 1-3, (1989)

[28]

Skinner D.Z., Camacho R.F., Genetic diversity within a potato leafhopper (Homoptera: Cicadellidae) population infesting alfalfa, Journal of Kansas Entomological Society, 68, pp. 35-42, (1995)

[29]

Stewart S.J., Excoffier L., Assessing population genetic structure and variability with RAPD data: Application to Vaccinium macrocarpon (American cranberry), Journal of Evolutionary Biology, 9, pp. 153-171, (1996)

[30]

Thormannosborn C.E.T.C., Use of RAPD and RFLP markers for germplasm evaluation, Applications of RAPD Technology to Plant Breeding, pp. 9-11, (1992)

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