First natural co-occurrence of tomato leaf curl New Delhi virus DNA-A and chili leaf curl betasatellite on tomato plants (Solanum lycopersicum L.) in India

被引：0
作者：
Aniruddha Kumar Agnihotri
Sudhakar Prasad Mishra
Ramesh Chandra Tripathi
Mohammad Ansar
Ashish Srivastava
Indra Prasad Tripathi
机构：
[1] Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalaya,Department of Crop Sciences, Faculty of Agriculture
[2] Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalaya,Department of Biological Sciences, Faculty of Science and Environment
[3] Bihar Agricultural University,Department of Plant Pathology
[4] Amity University,Amity Institute of Virology and Immunology
[5] University of Edinburgh,Institute of Molecular Plant Sciences
来源：
Journal of General Plant Pathology | 2018年 / 84卷
关键词：
Chili leaf curl betasatellite; PCR; Rolling circle amplification;
D O I：
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中图分类号：
学科分类号：
摘要：
Tomato leaf curl disease (ToLCD) affected 25% of the tomato crop in Chitrakoot, India and symptomatic leaves were collected for molecular assay. The complete sequences of bipartite begomovirus DNA-A and a betasatellite DNA were amplified. In a sequence analysis, begomovirus DNA-A and betasatellite shared highest sequence identity (91–99%) with Tomato leaf curl New Delhi virus (ToLCNDV) DNA-A and chili leaf curl betasatellite (ChLCB), respectively. The virus was transmitted by whitefly to tomato plants and caused ToLCD symptoms with 70% transmission rate. To our knowledge, this is the first report of the natural occurrence of ToLCNDV and ChLCB in India.
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页码：414 / 417
页数：3
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