c-Met-targeted RNA interference inhibits growth and metastasis of glioma U251 cells in vitro

被引:0
作者
Sheng-Hua Chu
Dong-Fu Feng
Hong Zhang
Er-Tao Chen
Zhi-Xin Duan
Xue-Yuan Li
Jia Li
Yan-Bin Ma
Zhi-An Zhu
Jian-Hua Qiu
机构
[1] No. 3 People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Department of Neurosurgery
来源
Journal of Neuro-Oncology | 2009年 / 93卷
关键词
c-Met; Apoptosis; Adherence; Gene therapy; Glioma; RNA interference;
D O I
暂无
中图分类号
学科分类号
摘要
Angiogenesis plays an essential role in tumor growth and metastasis and is a promising target for cancer therapy. c-Met, a receptor tyrosine kinase, and its ligand, hepatocyte growth factor (HGF), are critical in cellular proliferation, motility, invasion, and angiogenesis. The present study was designed to determine the role of c-Met in growth and metastasis of glioma U251 cells using RNA interference (RNAi) technology in vitro. We constructed three kinds of shRNA expression vectors aiming at the c-Met gene, then transfected them into glioma U251 cells by lipofectamineTM 2000. The level of c-Met mRNA was investigated by real-time polymerse chain reaction (RT-PCR). The protein expression of c-Met was observed by immunofluoresence staining and western blotting. U251 cell growth and adherence was detected by methyl thiazole tetrazolium assay. The apoptosis of U251 cells was examined with a flow cytometer. The adherence, invasion, and in vitro angiogenesis assays of U251 cells were done. We got three kinds of c-Met specific shRNA expression vectors which could efficiently inhibit the growth and metastasis of U251 cells and the expression of c-Met in U251 cells. RT-PCR, immunofluoresence staining and western blotting showed that inhibition rate for c-Met expression was up to 90%, 79% and 85%, respectively. The expression of c-Met can be inhibited by RNA interference in U251 cells, which can inhibit the growth and metastasis of U251 cell and induce cell apoptosis. These results indicate that RNAi of c-Met can be an effective antiangiogenic strategy for glioma.
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页码:183 / 189
页数:6
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